| Chapter title |
Marine Genomics
|
|---|---|
| Chapter number | 13 |
| Book title |
Marine Genomics
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3774-5_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3772-1, 978-1-4939-3774-5
|
| Authors |
Bourlat, Sarah J, Haenel, Quiterie, Finnman, Jennie, Leray, Matthieu, Sarah J. Bourlat, Quiterie Haenel, Jennie Finnman, Matthieu Leray |
| Editors |
Sarah J. Bourlat |
| Abstract |
This protocol details the preparation of multiplexed amplicon libraries for metabarcoding (amplicon-based) studies of microscopic marine eukaryotes. Metabarcoding studies, based on the amplification of a taxonomically informative marker from a collection of organisms or an environmental sample, can be performed to analyze biodiversity patterns or predator-prey interactions. For Metazoa, we use the mitochondrial cytochrome oxidase 1 (CO1) or the small ribosomal subunit (SSU) markers. Here, we describe a strategy for the preparation of multiplexed Illumina MiSeq libraries using a dual-PCR approach for the addition of index and adaptor sequences. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 20% |
| Unknown | 4 | 80% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 80% |
| Scientists | 1 | 20% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Unknown | 60 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 23% |
| Researcher | 9 | 15% |
| Student > Master | 6 | 10% |
| Student > Bachelor | 5 | 8% |
| Other | 4 | 7% |
| Other | 2 | 3% |
| Unknown | 21 | 34% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 30% |
| Environmental Science | 9 | 15% |
| Biochemistry, Genetics and Molecular Biology | 9 | 15% |
| Business, Management and Accounting | 1 | 2% |
| Earth and Planetary Sciences | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 22 | 36% |