Proteomics for Biomarker Discovery

Chapter 1 Pre- and Post-analytical Factors in Biomarker Discovery

Chapter 2 Pre-fractionation of Noncirculating Biological Fluids to Improve Discovery of Clinically Relevant Protein Biomarkers

Chapter 3 Serum Exosome Isolation by Size-Exclusion Chromatography for the Discovery and Validation of Preeclampsia-Associated Biomarkers

Chapter 5 A Standardized and Reproducible Proteomics Protocol for Bottom-Up Quantitative Analysis of Protein Samples Using SP3 and Mass Spectrometry

Chapter 6 Analyzing Cerebrospinal Fluid Proteomes to Characterize Central Nervous System Disorders: A Highly Automated Mass Spectrometry-Based Pipeline for Biomarker Discovery

Chapter 7 Lys-C/Trypsin Tandem-Digestion Protocol for Gel-Free Proteomic Analysis of Colon Biopsies

Chapter 8 Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics

Chapter 9 Protein Biomarker Discovery in Non-depleted Serum by Spectral Library-Based Data-Independent Acquisition Mass Spectrometry

Chapter 10 Discovering Protein Biomarkers from Clinical Peripheral Blood Mononuclear Cells Using Data-Independent Acquisition Mass Spectrometry

Chapter 11 Intact Protein Analysis by LC-MS for Characterizing Biomarkers in Cerebrospinal Fluid

Chapter 12 Detection of Proteoforms Using Top-Down Mass Spectrometry and Diagnostic Ions

Chapter 13 Development of a Highly Multiplexed SRM Assay for Biomarker Discovery in Formalin-Fixed Paraffin-Embedded Tissues

Chapter 14 Development and Validation of Multiple Reaction Monitoring (MRM) Assays for Clinical Applications

Chapter 15 Protein-Level Statistical Analysis of Quantitative Label-Free Proteomics Data with ProStaR

Chapter 16 Computation and Selection of Optimal Biomarker Combinations by Integrative ROC Analysis Using CombiROC

Chapter 17 PanelomiX for the Combination of Biomarkers

Chapter 18 Designing an In Silico Strategy to Select Tissue-Leakage Biomarkers Using the Galaxy Framework