Mammalian Oocyte Regulation
Humana Press
Chapter title |
Counting Chromosomes in Intact Eggs
|
---|---|
Chapter number | 17 |
Book title |
Mammalian Oocyte Regulation
|
Published in |
Methods in molecular biology, January 2013
|
DOI | 10.1007/978-1-62703-191-2_17 |
Pubmed ID | |
Book ISBNs |
978-1-62703-190-5, 978-1-62703-191-2
|
Authors |
Teresa Chiang, Michael A. Lampson |
Abstract |
Chromosomal spreads are an established method to assess ploidy in different cell types. However, many traditional chromosome-spreading techniques require dissolution of the cell and can only be used to assess hyperploidy because of potential chromosome loss inherent in the procedure. Here we describe a method to evaluate chromosome numbers in intact eggs so that both hyperploidy and hypoploidy can be accurately detected. |
Country | Count | As % |
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Unknown | 8 | 100% |
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 38% |
Other | 1 | 13% |
Student > Bachelor | 1 | 13% |
Student > Master | 1 | 13% |
Researcher | 1 | 13% |
Other | 1 | 13% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 4 | 50% |
Agricultural and Biological Sciences | 4 | 50% |