| Chapter title |
Incorporation of Non-Canonical Amino Acids
|
|---|---|
| Chapter number | 7 |
| Book title |
Novel Chemical Tools to Study Ion Channel Biology
|
| Published in |
Advances in experimental medicine and biology, January 2015
|
| DOI | 10.1007/978-1-4939-2845-3_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2844-6, 978-1-4939-2845-3
|
| Authors |
Lilia Leisle, Francis Valiyaveetil, Ryan A. Mehl, Christopher A. Ahern, Leisle, Lilia, Valiyaveetil, Francis, Mehl, Ryan A., Ahern, Christopher A. |
| Abstract |
In this chapter we discuss the strengths, caveats and technical considerations of three approaches for reprogramming the chemical composition of selected amino acids within a membrane protein. In vivo nonsense suppression in the Xenopus laevis oocyte, evolved orthogonal tRNA and aminoacyl-tRNA synthetase pairs and protein ligation for biochemical production of semisynthetic proteins have been used successfully for ion channel and receptor studies. The level of difficulty for the application of each approach ranges from trivial to technically demanding, yet all have untapped potential in their application to membrane proteins. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 66 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 24% |
| Student > Bachelor | 10 | 15% |
| Student > Master | 9 | 14% |
| Researcher | 8 | 12% |
| Student > Doctoral Student | 4 | 6% |
| Other | 6 | 9% |
| Unknown | 13 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 21 | 32% |
| Chemistry | 12 | 18% |
| Agricultural and Biological Sciences | 11 | 17% |
| Chemical Engineering | 1 | 2% |
| Computer Science | 1 | 2% |
| Other | 4 | 6% |
| Unknown | 16 | 24% |