| Chapter title |
Assembly of Multigene Constructs Using Golden Gate Cloning.
|
|---|---|
| Chapter number | 19 |
| Book title |
Glyco-Engineering
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2760-9_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2759-3, 978-1-4939-2760-9
|
| Authors |
Marillonnet, Sylvestre, Werner, Stefan, Sylvestre Marillonnet, Stefan Werner |
| Abstract |
Efficient DNA assembly methods are required for synthetic biology. Standardization of DNA parts is an essential element that not only facilitates reuse of the same parts for various constructs but also allows standardization of the assembly strategy. We provide here a protocol for assembly of multigene constructs from standard biological parts using the modular cloning system MoClo. Making constructs using this system requires to first define the structure of the final construct and to identify all basic parts and vectors required for the construction strategy. The cloning strategy is in large part determined by the structure of the final construct, which is then made using a series of one-pot Golden Gate cloning reactions. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Mexico | 1 | 2% |
| Unknown | 44 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 10 | 22% |
| Researcher | 8 | 17% |
| Student > Ph. D. Student | 7 | 15% |
| Student > Master | 5 | 11% |
| Professor | 3 | 7% |
| Other | 5 | 11% |
| Unknown | 8 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 22 | 48% |
| Agricultural and Biological Sciences | 9 | 20% |
| Chemical Engineering | 2 | 4% |
| Unspecified | 1 | 2% |
| Medicine and Dentistry | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 9 | 20% |