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Microinjection

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Cover of 'Microinjection'

Table of Contents

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    Book Overview
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    Chapter 1 Microinjection and Micromanipulation: A Historical Perspective
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    Chapter 2 Production of Transgenic Mice by Pronuclear Microinjection
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    Chapter 3 Transgene Recombineering in Bacterial Artificial Chromosomes
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    Chapter 4 Using TARGATT™ Technology to Generate Site-Specific Transgenic Mice
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    Chapter 5 Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method
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    Chapter 6 Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer
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    Chapter 7 Creating Knockin Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination Without Drug Selection
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    Chapter 8 Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice
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    Chapter 9 Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique
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    Chapter 10 Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation
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    Chapter 11 Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides
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    Chapter 12 Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection
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    Chapter 13 The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications
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    Chapter 14 The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm
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    Chapter 15 Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line
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    Chapter 16 Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology
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    Chapter 17 Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos
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    Chapter 18 Organ Generation from Knockedout Rat Blastocysts Complemented with Pluripotent Stem Cells
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    Chapter 19 Generation of Rabbit Models by Gene Editing Nucleases
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    Chapter 20 Production of Genetically Engineered Porcine Embryos by Handmade Cloning
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    Chapter 21 Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production
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    Chapter 22 Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer
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    Chapter 23 Generating Goat Mammary Gland Bioreactors for Producing Recombinant Proteins by Gene Targeting
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    Chapter 24 Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes
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    Chapter 25 Using Microinjection to Generate Genetically Modified Caenorhabditis elegans by CRISPR/Cas9 Editing
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    Chapter 26 Microinjection in Zebrafish for Genome Editing and Functional Studies
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    Chapter 27 Microinjection of Marine Fish Eggs
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    Chapter 28 Generating Gene Knockout Oryzias latipes and Rice Field Eel Using TALENs Method
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    Chapter 29 Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus
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    Chapter 30 Microinjection of Live Mammalian Cells: A Delivery Method that Provides Added Versatility to the Study of Cellular Function
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Title
Microinjection
Published by
Springer New York, December 2018
DOI 10.1007/978-1-4939-8831-0
ISBNs
978-1-4939-8830-3, 978-1-4939-8831-0
Editors

Liu, Chengyu, Du, Yubin

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