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Gene Correction

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Cover of 'Gene Correction'

Table of Contents

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    Book Overview
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    Chapter 1 RecTE Psy -Mediated Recombineering in Pseudomonas syringae
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    Chapter 2 Genome manipulations with bacterial recombineering and site-specific integration in Drosophila.
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    Chapter 3 Multiple Genetic Manipulations of DT40 Cell Line
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    Chapter 4 Gene Targeting of Human Pluripotent Stem Cells by Homologous Recombination
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    Chapter 5 Methods for the Assessment of ssODN-Mediated Gene Correction Frequencies in Muscle Cells.
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    Chapter 6 Small Fragment Homologous Replacement (SFHR): Sequence-Specific Modification of Genomic DNA in Eukaryotic Cells by Small DNA Fragments.
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    Chapter 7 Preparation and Application of Triple Helix Forming Oligonucleotides and Single Strand Oligonucleotide Donors for Gene Correction
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    Chapter 8 Triplex-Mediated Genome Targeting and Editing
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    Chapter 9 Targeting piggyBac Transposon Integrations in the Human Genome.
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    Chapter 10 Gene Targeting in Human-Induced Pluripotent Stem Cells with Adenoviral Vectors
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    Chapter 11 Enhanced Gene Targeting of Adult and Pluripotent Stem Cells Using Evolved Adeno-associated Virus
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    Chapter 12 Lentiviral Vectors Encoding Zinc-Finger Nucleases Specific for the Model Target Locus HPRT1
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    Chapter 13 Designing and Testing the Activities of TAL Effector Nucleases.
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    Chapter 14 A Bacterial One-Hybrid System to Isolate Homing Endonuclease Variants with Altered DNA Target Specificities
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    Chapter 15 Design and analysis of site-specific single-strand nicking endonucleases for gene correction.
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    Chapter 16 CRISPR-Cas-Mediated Targeted Genome Editing in Human Cells.
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    Chapter 17 RNA-Guided Genome Editing of Mammalian Cells
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    Chapter 18 Nuclease-Mediated Double-Strand Break (DSB) Enhancement of Small Fragment Homologous Recombination (SFHR) Gene Modification in Human-Induced Pluripotent Stem Cells (hiPSCs).
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    Chapter 19 AAV-Mediated Gene Editing via Double-Strand Break Repair
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    Chapter 20 Genetic Modification Stimulated by the Induction of a Site-Specific Break Distant from the Locus of Correction in Haploid and Diploid Yeast Saccharomyces cerevisiae
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    Chapter 21 A Southern Blot Protocol to Detect Chimeric Nuclease-Mediated Gene Repair
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    Chapter 22 High-Throughput Cellular Screening of Engineered Nuclease Activity Using the Single-Strand Annealing Assay and Luciferase Reporter
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    Chapter 23 An Unbiased Method for Detection of Genome-Wide Off-Target Effects in Cell Lines Treated with Zinc Finger Nucleases
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    Chapter 24 Identification of Off-Target Cleavage Sites of Zinc Finger Nucleases and TAL Effector Nucleases Using Predictive Models
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    Chapter 25 Method for Retinal Gene Repair in Neonatal Mouse
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    Chapter 26 In utero delivery of oligodeoxynucleotides for gene correction.
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    Chapter 27 Portal Vein Delivery of Viral Vectors for Gene Therapy for Hemophilia
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    Chapter 28 Gene Correction of Induced Pluripotent Stem Cells Derived from a Murine Model of X-Linked Chronic Granulomatous Disorder
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    Chapter 29 Efficient Transduction of Hematopoietic Stem Cells and Its Potential for Gene Correction of Hematopoietic Diseases
Attention for Chapter 13: Designing and Testing the Activities of TAL Effector Nucleases.
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Chapter title
Designing and Testing the Activities of TAL Effector Nucleases.
Chapter number 13
Book title
Gene Correction
Published in
Methods in molecular biology, February 2014
DOI 10.1007/978-1-62703-761-7_13
Pubmed ID
Book ISBNs
978-1-62703-760-0, 978-1-62703-761-7
Authors

Lin Y, Cradick TJ, Bao G, Yanni Lin, Thomas J. Cradick, Gang Bao, Lin, Yanni, Cradick, Thomas J., Bao, Gang

Abstract

Transcription activator-like effector nucleases (TALENs) have rapidly developed into a powerful tool for genome editing. To avoid labor-intensive and time-consuming experimental screening for active TALENs, a scoring system can help select optimal target sites. Here we describe a procedure to design active TALENs using a scoring system named Scoring Algorithm for Predicted TALEN Activity (SAPTA) and a method to test the activity of individual and pairs of TALENs.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 9%
South Africa 1 9%
Unknown 9 82%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 27%
Researcher 3 27%
Other 2 18%
Professor 1 9%
Student > Master 1 9%
Other 1 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 45%
Biochemistry, Genetics and Molecular Biology 3 27%
Economics, Econometrics and Finance 1 9%
Medicine and Dentistry 1 9%
Neuroscience 1 9%
Other 0 0%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 October 2014.
All research outputs
#20,238,443
of 22,765,347 outputs
Outputs from Methods in molecular biology
#9,865
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Outputs of similar age
#193,594
of 225,081 outputs
Outputs of similar age from Methods in molecular biology
#83
of 143 outputs
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So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 143 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.