Chapter title |
Cross-Linking Immunoprecipitation and qPCR (CLIP-qPCR) Analysis to Map Interactions Between Long Noncoding RNAs and RNA-Binding Proteins.
|
---|---|
Chapter number | 2 |
Book title |
Long Non-Coding RNAs
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3378-5_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3376-1, 978-1-4939-3378-5
|
Authors |
Yoon, Je-Hyun, Gorospe, Myriam, Je-Hyun Yoon, Myriam Gorospe |
Editors |
Yi Feng, Lin Zhang |
Abstract |
Mammalian cells express a wide range of transcripts, some protein-coding RNAs (mRNA) and many noncoding (nc) RNAs. Long (l)ncRNAscan modulates protein expression patterns by regulating gene transcription, pre-mRNA splicing, mRNA export, mRNA degradation, protein translation, and protein ubiquitination. Given the growing recognition that lncRNAs have a robust impact upon gene expression, there is rising interest in elucidating the levels and regulation of lncRNAs. A number of high-throughput methods have been developed recently to map the interaction of lncRNAs and RNA-binding proteins (RBPs). However, few of these approaches are suitable for mapping and quantifying RBP-lncRNA interactions. Here, we describe the recently developed method CLIP-qPCR (cross-linking and immunoprecipitation followed by reverse transcription and quantitative PCR) for mapping and quantifying RBP-lncRNA interactions. |
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Members of the public | 1 | 100% |
Mendeley readers
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Demographic breakdown
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Researcher | 6 | 11% |
Student > Master | 5 | 9% |
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Other | 2 | 4% |
Other | 6 | 11% |
Unknown | 16 | 29% |
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Unknown | 16 | 29% |