Chapter title |
Non-isotopic Method for In Situ LncRNA Visualization and Quantitation.
|
---|---|
Chapter number | 13 |
Book title |
Long Non-Coding RNAs
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3378-5_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3376-1, 978-1-4939-3378-5
|
Authors |
Maqsodi, Botoul, Nikoloff, Corina, Botoul Maqsodi, Corina Nikoloff |
Editors |
Yi Feng, Lin Zhang |
Abstract |
In mammals and other eukaryotes, most of the genome is transcribed in a developmentally regulated manner to produce large numbers of long noncoding RNAs (lncRNAs). Genome-wide studies have identified thousands of lncRNAs lacking protein-coding capacity. RNA in situ hybridization technique is especially beneficial for the visualization of RNA (mRNA and lncRNA) expression in a heterogeneous population of cells/tissues; however its utility has been hampered by complicated procedures typically developed and optimized for the detection of a specific gene and therefore not amenable to a wide variety of genes and tissues.Recently, bDNA has revolutionized RNA in situ detection with fully optimized, robust assays for the detection of any mRNA and lncRNA targets in formalin-fixed paraffin-embedded (FFPE) and fresh frozen tissue sections using manual processing. |
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