| Chapter title |
Interpretation Guidelines of a Standard Y-chromosome STR 17-plex PCR-CE Assay for Crime Casework.
|
|---|---|
| Chapter number | 4 |
| Book title |
DNA Electrophoresis Protocols for Forensic Genetics
|
| Published in |
Methods in molecular biology, January 2012
|
| DOI | 10.1007/978-1-61779-461-2_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-460-5, 978-1-61779-461-2
|
| Authors |
Lutz Roewer, Maria Geppert, Roewer, Lutz, Geppert, Maria |
| Abstract |
Y-STR analysis is an invaluable tool to examine evidence in sexual assault cases and in other forensic casework. Unambiguous detection of the male component in DNA mixtures with a high female background is still the main field of application of forensic Y-STR haplotyping. In the last years, powerful technologies including a 17-locus multiplex PCR assay have been introduced in the forensic laboratories. At the same time, statistical methods have been developed and adapted for interpretation of a nonrecombining, linear marker as the Y-chromosome which shows a strongly clustered geographical distribution due to the linear inheritance and the patrilocality of ancestral groups. Large population databases, namely the Y-STR Haplotype Reference Database (YHRD), have been established to assess the evidentiary value of Y-STR matches by means of frequency estimation methods (counting and extrapolation). |
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