| Chapter title |
Designed Methods for the Sorting of Tertiary Lymphoid Structure-Immune Cell Populations
|
|---|---|
| Chapter number | 11 |
| Book title |
Tertiary Lymphoid Structures
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8709-2_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8708-5, 978-1-4939-8709-2
|
| Authors |
Devi-Marulkar, Priyanka, Kaplon, Hélène, Dieu-Nosjean, Marie-Caroline, Lawand, Myriam, Priyanka Devi-Marulkar, Hélène Kaplon, Marie-Caroline Dieu-Nosjean, Myriam Lawand |
| Abstract |
The tumor microenvironment is a complex network of interacting cells composed of immune and nonimmune cells. It has been reported that the composition of the immune contexture has a significant impact on tumor growth and patient survival in different solid tumors. For instance, we and other groups have previously demonstrated that a strong infiltration of T-helper type 1 (Th1) or memory CD8+ T cells is associated with long-term survival of cancer patients. Nevertheless, the prognostic value of the other immune populations, namely regulatory T cells (Treg), B cells, and gamma delta (γδ) T cells, remains a matter of debate. Herein, we describe novel flow cytometry-based strategies to sort out these different immune populations in order to evaluate their role in non-small cell lung cancer (NSCLC). |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 14% |
| Student > Ph. D. Student | 1 | 14% |
| Student > Bachelor | 1 | 14% |
| Other | 1 | 14% |
| Student > Master | 1 | 14% |
| Other | 0 | 0% |
| Unknown | 2 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 2 | 29% |
| Biochemistry, Genetics and Molecular Biology | 2 | 29% |
| Unspecified | 1 | 14% |
| Unknown | 2 | 29% |