| Chapter title |
Measurement of Antibody-Mediated Reduction of Plasmodium yoelii Liver Burden by Bioluminescent Imaging.
|
|---|---|
| Chapter number | 6 |
| Book title |
Malaria Vaccines
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2815-6_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2814-9, 978-1-4939-2815-6
|
| Authors |
Sack, Brandon K, Miller, Jessica L, Vaughan, Ashley M, Kappe, Stefan H I, Brandon K. Sack, Jessica L. Miller, Ashley M. Vaughan, Stefan H. I. Kappe |
| Editors |
Ashley Vaughan |
| Abstract |
Antibodies against the infectious sporozoite stage of malaria have been shown to be effective in preventing infection of the liver and in mitigating the ensuing blood stage. However, only a handful of antibody targets have been vetted and shown to be successful in mediating in vivo protection. Even more limited are the means with which to measure how effectively antibodies can reduce the number of parasites establishing infection in the liver. Traditionally, only qPCR of infected mouse livers could accurately measure liver parasite burden. However, this procedure requires sacrifice of the animal and precludes monitoring of the ensuing blood stage infection. Herein we describe a method of accurately assessing antibody-mediated reduction of parasite liver burden by combining passive or active immunization of mice and mosquito bite challenge with luciferase-expressing transgenic P. yoelii parasites. This method is rapid, reliable and allows for observation of blood stage disease in the same animal. This model will prove integral in screening the efficacy of novel antibody targets as the search for a more effective malaria vaccine continues. |
X Demographics
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|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 36% |
| Researcher | 2 | 18% |
| Student > Bachelor | 1 | 9% |
| Other | 1 | 9% |
| Student > Doctoral Student | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 1 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 3 | 27% |
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| Nursing and Health Professions | 1 | 9% |
| Veterinary Science and Veterinary Medicine | 1 | 9% |
| Economics, Econometrics and Finance | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 2 | 18% |