↓ Skip to main content
What is this page? Embed badge

Malaria Vaccines

Overview of attention for book
Cover of 'Malaria Vaccines'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Isolation of Non-parenchymal Cells from the Mouse Liver.
  3. Altmetric Badge
    Chapter 2 Measurement of the T Cell Response to Preerythrocytic Vaccination in Mice.
  4. Altmetric Badge
    Chapter 3 Characterization of Liver CD8 T Cell Subsets that are Associated with Protection Against Pre-erythrocytic Plasmodium Parasites.
  5. Altmetric Badge
    Chapter 4 Flow Cytometry-Based Assessment of Antibody Function Against Malaria Pre-erythrocytic Infection.
  6. Altmetric Badge
    Chapter 5 Assessment of Parasite Liver-Stage Burden in Human-Liver Chimeric Mice
  7. Altmetric Badge
    Chapter 6 Measurement of Antibody-Mediated Reduction of Plasmodium yoelii Liver Burden by Bioluminescent Imaging.
  8. Altmetric Badge
    Chapter 7 Detection of Plasmodium berghei and Plasmodium yoelii Liver-Stage Parasite Burden by Quantitative Real-Time PCR.
  9. Altmetric Badge
    Chapter 8 Membrane Feeding Assay to Determine the Infectiousness of Plasmodium vivax Gametocytes.
  10. Altmetric Badge
    Chapter 9 The Standard Membrane Feeding Assay: Advances Using Bioluminescence.
  11. Altmetric Badge
    Chapter 10 Agglutination Assays of the Plasmodium falciparum-Infected Erythrocyte.
  12. Altmetric Badge
    Chapter 11 Antibody-Dependent Cell-Mediated Inhibition (ADCI) of Plasmodium falciparum: One- and Two-Step ADCI Assays.
  13. Altmetric Badge
    Chapter 12 A Robust Phagocytosis Assay to Evaluate the Opsonic Activity of Antibodies against Plasmodium falciparum-Infected Erythrocytes.
  14. Altmetric Badge
    Chapter 13 Miniaturized Growth Inhibition Assay to Assess the Anti-blood Stage Activity of Antibodies.
  15. Altmetric Badge
    Chapter 14 Measuring Plasmodium falciparum Erythrocyte Invasion Phenotypes Using Flow Cytometry.
  16. Altmetric Badge
    Chapter 15 The In Vitro Invasion Inhibition Assay (IIA) for Plasmodium vivax.
  17. Altmetric Badge
    Chapter 16 The Ex Vivo IFN-γ Enzyme-Linked Immunospot (ELISpot) Assay.
  18. Altmetric Badge
    Chapter 17 Evaluating IgG Antibody to Variant Surface Antigens Expressed on Plasmodium falciparum Infected Erythrocytes Using Flow Cytometry.
  19. Altmetric Badge
    Chapter 18 Inhibition of Infected Red Blood Cell Binding to the Vascular Endothelium.
  20. Altmetric Badge
    Chapter 19 Evaluation of Pregnancy Malaria Vaccine Candidates: The Binding Inhibition Assay.
  21. Altmetric Badge
    Chapter 20 High-Throughput Testing of Antibody-Dependent Binding Inhibition of Placental Malaria Parasites.
  22. Altmetric Badge
    Chapter 21 Generation of Transgenic Rodent Malaria Parasites Expressing Human Malaria Parasite Proteins.
  23. Altmetric Badge
    Chapter 22 Vaccination Using Gene-Gun Technology.
Attention for Chapter 7: Detection of Plasmodium berghei and Plasmodium yoelii Liver-Stage Parasite Burden by Quantitative Real-Time PCR.
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
2 X users

Readers on

mendeley
16 Mendeley
Summary X
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Detection of Plasmodium berghei and Plasmodium yoelii Liver-Stage Parasite Burden by Quantitative Real-Time PCR.
Chapter number 7
Book title
Malaria Vaccines
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2815-6_7
Pubmed ID
26450381
Book ISBNs
978-1-4939-2814-9, 978-1-4939-2815-6
Authors

Pichugin, Alexander, Krzych, Urszula, Alexander Pichugin, Urszula Krzych

Editors

Ashley Vaughan

Abstract

Direct detection and quantification of liver-stage Plasmodium parasites became possible with the development of quantitative real-time PCR (qPCR). Here we describe the measurement of parasite burden in the livers of mice infected with the rodent malaria species, Plasmodium berghei and Plasmodium yoelii. This method is based on detection of expression of parasite ribosomal 18S RNA and can serve as an endpoint to accurately evaluate the efficacy of vaccines targeting the preerythrocytic stages of malaria. This approach is fast and highly reproducible and allows quantification of liver-stage parasite burden in different mouse strains and different Plasmodium species after infection with a range of sporozoite challenge doses.

View on publisher site
X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 5 31%
Student > Doctoral Student 3 19%
Student > Ph. D. Student 2 13%
Student > Bachelor 2 13%
Lecturer > Senior Lecturer 1 6%
Other 2 13%
Unknown 1 6%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 19%
Agricultural and Biological Sciences 3 19%
Medicine and Dentistry 2 13%
Immunology and Microbiology 2 13%
Veterinary Science and Veterinary Medicine 1 6%
Other 3 19%
Unknown 2 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 October 2015.
All research outputs
#18,428,159
of 22,829,683 outputs
Outputs from Methods in molecular biology
#7,918
of 13,125 outputs
Outputs of similar age
#255,898
of 353,161 outputs
Outputs of similar age from Methods in molecular biology
#481
of 997 outputs
Altmetric has tracked 22,829,683 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,125 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,161 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 997 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.

This page is provided by Altmetric.