Chapter title |
Isolation of Non-parenchymal Cells from the Mouse Liver.
|
---|---|
Chapter number | 1 |
Book title |
Malaria Vaccines
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2815-6_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2814-9, 978-1-4939-2815-6
|
Authors |
Mohar, Isaac, Brempelis, Katherine J, Murray, Sara A, Ebrahimkhani, Mohammad R, Crispe, I Nicholas, Isaac Mohar, Katherine J. Brempelis, Sara A. Murray, Mohammad R. Ebrahimkhani, I. Nicholas Crispe |
Editors |
Ashley Vaughan |
Abstract |
Hepatocytes comprise the majority of liver mass and cell number. However, in order to understand liver biology, the non-parenchymal cells (NPCs) must be considered. Herein, a relatively rapid and efficient method for isolating liver NPCs from a mouse is described. Using this method, liver sinusoidal endothelial cells, Kupffer cells, natural killer (NK) and NK-T cells, dendritic cells, CD4+ and CD8+ T cells, and quiescent hepatic stellate cells can be purified. This protocol permits the collection of peripheral blood, intact liver tissue, and hepatocytes, in addition to NPCs. In situ perfusion via the portal vein leads to efficient liver digestion. NPCs are enriched from the resulting single-cell suspension by differential and gradient centrifugation. The NPCs can by analyzed or sorted into highly enriched populations using flow cytometry. The isolated cells are suitable for flow cytometry, protein, and mRNA analyses as well as primary culture. |
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