Chapter title |
Controlled Assembly of the Filamentous Chaperone Gamma-Prefoldin into Defined Nanostructures
|
---|---|
Chapter number | 22 |
Book title |
Protein Scaffolds
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7893-9_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7892-2, 978-1-4939-7893-9
|
Authors |
Douglas S. Clark, Dominic J. Glover, Clark, Douglas S., Glover, Dominic J. |
Abstract |
Self-assembling protein templates have enormous potential for the fabrication of multifunctional nanostructures that require precise positioning of individual molecules, such as enzymes and inorganic moieties, in regular patterns. A recently described approach uses ultrastable filaments composed of the gamma-prefoldin (γPFD) protein and engineered connector proteins to construct novel architectures useful for basic research and practical applications in nanobiotechnology. Here we describe the production of the γPFD and connector proteins from E. coli, and the assembly of γPFD with connector proteins into macromolecular structures with defined shapes. |
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