Chapter title |
Purification of Fungal High Molecular Weight Genomic DNA from Environmental Samples
|
---|---|
Chapter number | 3 |
Book title |
Fungal Genomics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7804-5_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7803-8, 978-1-4939-7804-5
|
Authors |
Laure Fauchery, Stéphane Uroz, Marc Buée, Annegret Kohler, Fauchery, Laure, Uroz, Stéphane, Buée, Marc, Kohler, Annegret |
Abstract |
Sequencing of a high number of fungal genomes has become possible due to the development of next generation sequencing techniques (NGS). The most recent developments aim to sequence single-molecule long-reads in order to improve genome assemblies, but consequently needs higher quality (minimum >20 kbp) DNA as starting material. However, environmental-derived samples from soil, wood, or litter often contain phenolic compounds, pigments, and other molecules that can be inhibitors for reactions during sequencing library construction. In this chapter, we propose an optimized protocol allowing the preparation of high quality and long fragment DNA from different samples (mycelium, fruiting body, soil) compatible with the current sequencing requirements. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 21% |
Student > Master | 2 | 14% |
Unspecified | 1 | 7% |
Other | 1 | 7% |
Researcher | 1 | 7% |
Other | 2 | 14% |
Unknown | 4 | 29% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 4 | 29% |
Biochemistry, Genetics and Molecular Biology | 3 | 21% |
Environmental Science | 1 | 7% |
Unspecified | 1 | 7% |
Immunology and Microbiology | 1 | 7% |
Other | 0 | 0% |
Unknown | 4 | 29% |