Chapter title |
Active Induction of Experimental Autoimmune Encephalomyelitis (EAE) with MOG35–55 in the Mouse
|
---|---|
Chapter number | 17 |
Book title |
Myelin
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7862-5_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7861-8, 978-1-4939-7862-5
|
Authors |
Mercedes Giralt, Amalia Molinero, Juan Hidalgo, Giralt, Mercedes, Molinero, Amalia, Hidalgo, Juan |
Abstract |
Experimental autoimmune encephalomyelitis (EAE) is one of the most popular animal models of multiple sclerosis (MS). There are a number of EAE models, being actively induced EAE in strains such as C57Bl/6 mice very robust and reproducible. We herewith present details of the materials and methods for active EAE. Mice are immunized with an emulsion of myelin oligodendrocyte glycoprotein peptide 35-55 (MOG35-55) + complete Freund's adjuvant (supplemented with Mycobacterium tuberculosis), and treated with Bordetella pertussis toxin, to induce EAE. Sham-EAE mice are immunized with bovine serum albumin instead of MOG35-55. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 52 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 15% |
Student > Bachelor | 8 | 15% |
Student > Master | 5 | 10% |
Student > Doctoral Student | 4 | 8% |
Researcher | 4 | 8% |
Other | 5 | 10% |
Unknown | 18 | 35% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 7 | 13% |
Biochemistry, Genetics and Molecular Biology | 6 | 12% |
Agricultural and Biological Sciences | 5 | 10% |
Immunology and Microbiology | 5 | 10% |
Medicine and Dentistry | 4 | 8% |
Other | 5 | 10% |
Unknown | 20 | 38% |