Chapter title |
Modeling PNS and CNS Myelination Using Microfluidic Chambers
|
---|---|
Chapter number | 12 |
Book title |
Myelin
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7862-5_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7861-8, 978-1-4939-7862-5
|
Authors |
Adrien Vaquié, Alizée Sauvain, Claire Jacob, Vaquié, Adrien, Sauvain, Alizée, Jacob, Claire |
Abstract |
Modeling myelination in vitro allows mechanistic study of developmental myelination and short-term myelin maintenance, but analyses possible to carry out using currently available models are usually limited because of high cell density and the lack of separation between neurons and myelinating cells. Furthermore, regeneration studies of myelinated systems after lesion require compartmentalization of neuronal cell bodies, axons, and myelinating cells. Here we describe a compartmentalized method using microfluidics that allows live-cell imaging at the single-cell level to follow short- and long-term dynamic interactions of neurons and myelinating cells and large-scale analyses, e.g., RNA sequencing on pure or highly enriched neurons or myelinating cells, separately. |
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