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Chapter title |
Isolation and Culture of Juvenile Pig Thyroid Follicular Epithelia
|
---|---|
Chapter number | 2 |
Book title |
Epithelial Cell Culture
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8600-2_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8599-9, 978-1-4939-8600-2
|
Authors |
James D. Lillich, Peying Fong, Lillich, James D., Fong, Peying |
Abstract |
Epithelial tissues are defined by their polarity and their ability to transport directionally. Thyroid is a tissue comprising functional epithelial units organized as enclosed follicles, with their luminal spaces defined by thyrocyte apices. Thus, the native arrangement of thyroid epithelia limits accessibility to the follicular space, presenting a challenge in studying transepithelial movements. This limitation can be overcome by studying thyrocytes grown as two-dimensional cultures. Herein we present methods for isolation of thyroid follicles from juvenile pigs and preparation of high-resistance, polarized cultures. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 33% |
Student > Master | 1 | 33% |
Professor > Associate Professor | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 33% |
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Medicine and Dentistry | 1 | 33% |