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Epithelial Cell Culture

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Cover of 'Epithelial Cell Culture'

Table of Contents

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    Book Overview
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    Chapter 1 Normal Human Thyrocytes in Culture
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    Chapter 2 Isolation and Culture of Juvenile Pig Thyroid Follicular Epithelia
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    Chapter 3 Reassembly of Functional Human Stem/Progenitor Cells in 3D Culture
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    Chapter 4 Culture and Differentiation of Lung Bronchiolar Epithelial Cells In Vitro
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    Chapter 5 Differentiation of Gastrointestinal Cell Lines by Culture in Semi-wet Interface
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    Chapter 6 Three-Dimensional Cell Culture Model Utilization in Renal Carcinoma Cancer Stem Cell Research
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    Chapter 7 Amniotic Epithelial Cell Culture
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    Chapter 8 Bovine Granulosa Cell Culture
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    Chapter 9 Bioencapsulation of Oocytes and Granulosa Cells
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    Chapter 10 Ovine Granulosa Cells Isolation and Culture to Improve Oocyte Quality
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    Chapter 11 3D Model Replicating the Intestinal Function to Evaluate Drug Permeability
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    Chapter 12 Isolation of Human Gastric Epithelial Cells from Gastric Surgical Tissue and Gastric Biopsies for Primary Culture
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    Chapter 13 Long-Term Culture of Intestinal Organoids
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    Chapter 14 Bovine Mammary Organoids: A Model to Study Epithelial Mammary Cells
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    Chapter 15 Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from Organoids
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    Chapter 16 Mouse-Derived Gastric Organoid and Immune Cell Co-culture for the Study of the Tumor Microenvironment
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    Chapter 17 Murine and Human Mammary Cancer Cell Lines: Functional Tests
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    Chapter 18 In Vitro Porcine Colon Culture
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    Chapter 19 Primary Cultures of Olfactory Neurons from the Avian Olfactory Epithelium
Attention for Chapter 2: Isolation and Culture of Juvenile Pig Thyroid Follicular Epithelia
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Chapter title
Isolation and Culture of Juvenile Pig Thyroid Follicular Epithelia
Chapter number 2
Book title
Epithelial Cell Culture
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8600-2_2
Pubmed ID
Book ISBNs
978-1-4939-8599-9, 978-1-4939-8600-2
Authors

James D. Lillich, Peying Fong, Lillich, James D., Fong, Peying

Abstract

Epithelial tissues are defined by their polarity and their ability to transport directionally. Thyroid is a tissue comprising functional epithelial units organized as enclosed follicles, with their luminal spaces defined by thyrocyte apices. Thus, the native arrangement of thyroid epithelia limits accessibility to the follicular space, presenting a challenge in studying transepithelial movements. This limitation can be overcome by studying thyrocytes grown as two-dimensional cultures. Herein we present methods for isolation of thyroid follicles from juvenile pigs and preparation of high-resistance, polarized cultures.

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Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 33%
Student > Master 1 33%
Professor > Associate Professor 1 33%
Readers by discipline Count As %
Unspecified 1 33%
Biochemistry, Genetics and Molecular Biology 1 33%
Medicine and Dentistry 1 33%