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Mendeley readers
Chapter title |
High-Resolution Single-Molecule Kinesin Assays at kHz Frame Rates
|
---|---|
Chapter number | 7 |
Book title |
Molecular Motors
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8556-2_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8554-8, 978-1-4939-8556-2
|
Authors |
Keith J. Mickolajczyk, William O. Hancock, Mickolajczyk, Keith J., Hancock, William O. |
Abstract |
This chapter describes methods for high-speed, unloaded, in vitro single-molecule kinesin tracking experiments. Instructions are presented for constructing a total internal reflection dark-field microscope (TIRDFM) and labeling motors with gold nanoparticles. An AMP-PNP unlocking assay is introduced as a specialized means of capturing processive events in a reduced field of view. Finally, step-finding tools for analyzing high frame-rate tracking data are described. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 30% |
Researcher | 3 | 30% |
Student > Bachelor | 2 | 20% |
Professor | 1 | 10% |
Unknown | 1 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 30% |
Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Physics and Astronomy | 2 | 20% |
Chemistry | 1 | 10% |
Engineering | 1 | 10% |
Other | 0 | 0% |
Unknown | 1 | 10% |