Chapter title |
The Role of Supercoiling in the Motor Activity of RNA Polymerases
|
---|---|
Chapter number | 11 |
Book title |
Molecular Motors
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8556-2_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8554-8, 978-1-4939-8556-2
|
Authors |
Annick Lesne, Jean-Marc Victor, Edouard Bertrand, Eugenia Basyuk, Maria Barbi, Lesne, Annick, Victor, Jean-Marc, Bertrand, Edouard, Basyuk, Eugenia, Barbi, Maria |
Abstract |
RNA polymerase (RNAP) is, in its elongation phase, an emblematic example of a molecular motor whose activity is highly sensitive to DNA supercoiling. After a review of DNA supercoiling basic features, we discuss how supercoiling controls polymerase velocity, while being itself modified by polymerase activity. This coupling is supported by single-molecule measurements. Physical modeling allows us to describe quantitatively how supercoiling and torsional constraints mediate a mechanical coupling between adjacent polymerases. On this basis, we obtain a description that may explain the existence and functioning of RNAP convoys. |
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Unknown | 2 | 25% |
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Unknown | 2 | 25% |