Fungal Genomics
Springer New York
Chapter title |
Mass Spectrometry-Based Proteomics
|
---|---|
Chapter number | 9 |
Book title |
Fungal Genomics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7804-5_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7803-8, 978-1-4939-7804-5
|
Authors |
Marcos Rafael Di Falco, Di Falco, Marcos Rafael |
Abstract |
Proteomics is the large-scale analysis of proteins rendered possible by modern mass spectrometry analysis methods capable of identifying thousands of peptides/proteins in a fast high-throughput manner. Here I describe protocols for the preparation of fungal culture protein samples for mass spectrometry-based proteomics analysis including protein sample cleanup, proteolytic digestion, LC-MS/MS separation, and database search protein identification. |
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