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Mendeley readers
Chapter title |
Loop-Mediated Isothermal Amplification (LAMP) for Detection of Phytoplasmas in the Field
|
---|---|
Chapter number | 8 |
Book title |
Plant Pathology
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2620-6_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2619-0, 978-1-4939-2620-6
|
Authors |
Matt Dickinson |
Abstract |
Loop-mediated isothermal amplification (LAMP) is a nucleic acid-based detection method with many applications. This chapter details its use for detection of phytoplasma diseases, combining a rapid 2-min DNA extraction method with real-time LAMP product detection such that the entire procedure can be undertaken and completed in the field within 40 min. Furthermore, the assays include an anneal curve validation step to guard against false positives and an assay for host plant DNA to guard against false negatives. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Malaysia | 1 | 6% |
Unknown | 17 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 33% |
Researcher | 4 | 22% |
Student > Bachelor | 2 | 11% |
Other | 2 | 11% |
Student > Master | 1 | 6% |
Other | 1 | 6% |
Unknown | 2 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 8 | 44% |
Biochemistry, Genetics and Molecular Biology | 5 | 28% |
Unspecified | 1 | 6% |
Immunology and Microbiology | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Other | 0 | 0% |
Unknown | 2 | 11% |