Chapter title |
Highly Efficient and Reliable DNA Aptamer Selection Using the Partitioning Capabilities of ddPCR: The Hi-Fi SELEX Method
|
---|---|
Chapter number | 30 |
Book title |
Digital PCR
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7778-9_30 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7776-5, 978-1-4939-7778-9
|
Authors |
Aaron Ang, Eric Ouellet, Karen C. Cheung, Charles Haynes, Ang, Aaron, Ouellet, Eric, Cheung, Karen C., Haynes, Charles |
Abstract |
In addition to its growing use in detecting and quantifying genes and larger genomic events, the partitioning used in digital PCR can serve as a powerful tool for high-fidelity amplification of synthetic combinatorial libraries of single-stranded DNA. Sequence-diverse libraries of this type are used as a basis for selecting tight-binding aptamers against a specific target. Here we provide a detailed description of the Hi-Fi SELEX protocol for rapid and efficient DNA aptamer selection. As part of that methodology, we describe how Hi-Fi SELEX gains advantages over other aptamer selection methods in part through the use of the massive partitioning capability of digital PCR. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 4 | 17% |
Researcher | 3 | 13% |
Student > Ph. D. Student | 3 | 13% |
Other | 1 | 4% |
Student > Doctoral Student | 1 | 4% |
Other | 3 | 13% |
Unknown | 8 | 35% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 35% |
Unspecified | 4 | 17% |
Computer Science | 1 | 4% |
Immunology and Microbiology | 1 | 4% |
Engineering | 1 | 4% |
Other | 0 | 0% |
Unknown | 8 | 35% |