↓ Skip to main content
What is this page? Embed badge

Digital PCR

Overview of attention for book
Cover of 'Digital PCR'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Entering the Pantheon of 21 st Century Molecular Biology Tools: A Perspective on Digital PCR
  3. Altmetric Badge
    Chapter 2 Basic Concepts and Validation of Digital PCR Measurements
  4. Altmetric Badge
    Chapter 3 Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies–What Does It Mean?
  5. Altmetric Badge
    Chapter 4 Control Materials and Digital PCR Methods for Evaluation of Circulating Cell-Free DNA Extractions from Plasma
  6. Altmetric Badge
    Chapter 5 Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize Events
  7. Altmetric Badge
    Chapter 6 Using Droplet Digital PCR to Detect Coinfection of Human Herpesviruses 6A and 6B (HHV-6A and HHV-6B) in Clinical Samples
  8. Altmetric Badge
    Chapter 7 Biomarkers in Cerebrospinal Fluid: Analysis of Cell-Free Circulating Mitochondrial DNA by Digital PCR
  9. Altmetric Badge
    Chapter 8 Testing of General and Human-Associated Fecal Contamination in Waters
  10. Altmetric Badge
    Chapter 9 Analyzing Copy Number Variation with Droplet Digital PCR
  11. Altmetric Badge
    Chapter 10 Assessing HER2 Amplification in Plasma cfDNA
  12. Altmetric Badge
    Chapter 11 Detection and Quantification of Mosaic Genomic DNA Variation in Primary Somatic Tissues Using ddPCR: Analysis of Mosaic Transposable-Element Insertions, Copy-Number Variants, and Single-Nucleotide Variants
  13. Altmetric Badge
    Chapter 12 Monitoring of Response and Resistance in Plasma of EGFR -Mutant Lung Cancer Using Droplet Digital PCR
  14. Altmetric Badge
    Chapter 13 Detection of Cancer DNA in Early Stage and Metastatic Breast Cancer Patients
  15. Altmetric Badge
    Chapter 14 Droplet Digital PCR for Minimal Residual Disease Detection in Mature Lymphoproliferative Disorders
  16. Altmetric Badge
    Chapter 15 Quantitation of JAK2 V617F Allele Burden by Using the QuantStudio™ 3D Digital PCR System
  17. Altmetric Badge
    Chapter 16 Novel Multiplexing Strategies for Quantification of Rare Alleles Using ddPCR
  18. Altmetric Badge
    Chapter 17 Identification and Use of Personalized Genomic Markers for Monitoring Circulating Tumor DNA
  19. Altmetric Badge
    Chapter 18 Single Color Multiplexed ddPCR Copy Number Measurements and Single Nucleotide Variant Genotyping
  20. Altmetric Badge
    Chapter 19 A Universal Droplet Digital PCR Approach for Monitoring of Graft Health After Transplantation Using a Preselected SNP Set
  21. Altmetric Badge
    Chapter 20 Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci Using Droplet Digital PCR
  22. Altmetric Badge
    Chapter 21 DNA Methylation Analysis Using Droplet Digital PCR
  23. Altmetric Badge
    Chapter 22 Simultaneous Quantification of Multiple Alternatively Spliced mRNA Transcripts Using Droplet Digital PCR
  24. Altmetric Badge
    Chapter 23 Using Droplet Digital PCR to Analyze Allele-Specific RNA Expression
  25. Altmetric Badge
    Chapter 24 Very Low Abundance Single-Cell Transcript Quantification with 5-Plex ddPCRTM Assays
  26. Altmetric Badge
    Chapter 25 Quantification of Circulating MicroRNAs by Droplet Digital PCR
  27. Altmetric Badge
    Chapter 26 Droplet Digital PCR for Absolute Quantification of Extracellular MicroRNAs in Plasma and Serum: Quantification of the Cancer Biomarker hsa-miR-141
  28. Altmetric Badge
    Chapter 27 Droplet Digital™ PCR Next-Generation Sequencing Library QC Assay
  29. Altmetric Badge
    Chapter 28 Phasing DNA Markers Using Digital PCR
  30. Altmetric Badge
    Chapter 29 ddTRAP: A Method for Sensitive and Precise Quantification of Telomerase Activity
  31. Altmetric Badge
    Chapter 30 Highly Efficient and Reliable DNA Aptamer Selection Using the Partitioning Capabilities of ddPCR: The Hi-Fi SELEX Method
Attention for Chapter 29: ddTRAP: A Method for Sensitive and Precise Quantification of Telomerase Activity
Altmetric Badge

About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (55th percentile)
  • High Attention Score compared to outputs of the same age and source (83rd percentile)

Mentioned by

wikipedia
1 Wikipedia page

Readers on

mendeley
39 Mendeley
Summary Wikipedia
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
ddTRAP: A Method for Sensitive and Precise Quantification of Telomerase Activity
Chapter number 29
Book title
Digital PCR
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7778-9_29
Pubmed ID
29717462
Book ISBNs
978-1-4939-7776-5, 978-1-4939-7778-9
Authors

Andrew T. Ludlow, Dawne Shelton, Woodring E. Wright, Jerry W. Shay, Ludlow, Andrew T., Shelton, Dawne, Wright, Woodring E., Shay, Jerry W.

Abstract

Telomerase is a cellular RNA template-dependent reverse transcriptase that adds telomere repeats to the 3' ends of chromosomes. Telomerase is expressed almost universally in tumor cells (>85%) to maintain telomere length, thus providing the ability of tumor cells to avoid senescence and to have unlimited replication ability, one of the key hallmarks of cancer. ddTRAP (droplet digital Telomere Repeat Amplification Protocol) is a two-step assay with whole cell lysates that utilizes a telomerase-mediated primer extension followed by droplet digital PCR (ddPCR) detection of extended products. The adoptation of the TRAP assay to ddPCR has resulted in improved throughput, increased sensitivity and better repeatability of the TRAP assay. The protocol described below details our procedures for ddTRAP.

View on publisher site
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 39 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 21%
Student > Master 6 15%
Student > Bachelor 3 8%
Professor 2 5%
Student > Postgraduate 2 5%
Other 5 13%
Unknown 13 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 13 33%
Agricultural and Biological Sciences 3 8%
Pharmacology, Toxicology and Pharmaceutical Science 2 5%
Medicine and Dentistry 2 5%
Business, Management and Accounting 1 3%
Other 3 8%
Unknown 15 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 July 2019.
All research outputs
#7,571,329
of 23,090,520 outputs
Outputs from Methods in molecular biology
#2,350
of 13,206 outputs
Outputs of similar age
#153,836
of 442,634 outputs
Outputs of similar age from Methods in molecular biology
#230
of 1,499 outputs
Altmetric has tracked 23,090,520 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,206 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,634 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.
We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.

This page is provided by Altmetric.