Chapter title |
Imaging and Sensing of Cadmium in Cells
|
---|---|
Chapter number | 5 |
Book title |
Cadmium: From Toxicity to Essentiality
|
Published in |
Metal ions in life sciences, February 2016
|
DOI | 10.1007/978-94-007-5179-8_5 |
Pubmed ID | |
Book ISBNs |
978-9-40-075178-1, 978-9-40-075179-8
|
Authors |
Masayasu Taki |
Abstract |
Cadmium is one of the highly toxic transition metals for human beings and is known as a human carcinogen. Once humans are exposed to Cd(2+) on a chronic basis, Cd(2+) primarily accumulates in the liver and kidney where it forms complexes with small peptides and proteins via sulfhydryl groups. Complexed Cd(2+) or the ionic Cd(2+) is then taken up by target cells and tissues and exerts the toxicity. However, the question of how non-essential Cd(2+) crosses the cell membranes remains unanswered. Furthermore, the molecular mechanism of Cd(2+)-induced physiological signaling disruption in cells is still not fully elucidated. Investigations of Cd(2+) uptake kinetics, distributions, and concentrations in cells require chemical tools for its detection. Because of the easy use and high spatiotemporal resolution, optical imaging using fluorescence microscopy is a well-suited method for monitoring Cd(2+) in biological samples. This chapter summarizes design principles of small molecule fluorescent sensors for Cd(2+) detection in aqueous solution and their photophysical and metal-binding properties. Also the applications of probes for fluorescence imaging of Cd(2+) in a variety of cell types are demonstrated. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 29 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 8 | 28% |
Student > Bachelor | 4 | 14% |
Researcher | 4 | 14% |
Student > Ph. D. Student | 3 | 10% |
Professor > Associate Professor | 2 | 7% |
Other | 1 | 3% |
Unknown | 7 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 6 | 21% |
Agricultural and Biological Sciences | 6 | 21% |
Environmental Science | 2 | 7% |
Earth and Planetary Sciences | 2 | 7% |
Biochemistry, Genetics and Molecular Biology | 1 | 3% |
Other | 5 | 17% |
Unknown | 7 | 24% |