Chapter title |
Manipulating the fragile x mental retardation proteins in the frog.
|
---|---|
Chapter number | 9 |
Book title |
Modeling Fragile X Syndrome
|
Published in |
Results and problems in cell differentiation, January 2012
|
DOI | 10.1007/978-3-642-21649-7_9 |
Pubmed ID | |
Book ISBNs |
978-3-64-221648-0, 978-3-64-221649-7
|
Authors |
Marc-Etienne Huot, Nicolas Bisson, Thomas Moss, Edouard W. Khandjian, Huot, Marc-Etienne, Bisson, Nicolas, Moss, Thomas, Khandjian, Edouard W. |
Abstract |
The frog is a model of choice to study gene function during early development, since a large number of eggs are easily obtained and rapidly develop external to the mother. This makes it a highly flexible model system in which direct tests of gene function can be investigated by microinjecting RNA antisense reagents. Two members of the Fragile X Related (FXR) gene family, namely xFmr1 and xFxr1 have been identified in Xenopus. While the tissue distribution of their products was found to be identical to that in mammals, the pattern of isoform expression is less complex. Translational silencing of the xFmr1 and xFxr1 mRNAs by microinjection of antisense morpholino oligonucleotides (MO) induced dramatic morphological alterations, revealing tissue-specific requirements for each protein during development and in maintaining the steady state levels of a range of transcripts in these tissues. The power and versatility of the frog model is that the MO-induced phenotypes can be rescued by microinjection of the corresponding MO-insensitive mRNAs. Most importantly, this animal model allows one rapidly to determine whether any member of the FXR family can compensate for the absence of another, an approach that cannot be performed in other animal models. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 22% |
Student > Master | 2 | 22% |
Professor | 1 | 11% |
Other | 1 | 11% |
Student > Bachelor | 1 | 11% |
Other | 1 | 11% |
Unknown | 1 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 56% |
Biochemistry, Genetics and Molecular Biology | 3 | 33% |
Unknown | 1 | 11% |