| Chapter title |
Genome-Wide RNAi Screening to Identify Regulators of Oncogene-Induced Cellular Senescence
|
|---|---|
| Chapter number | 25 |
| Book title |
Cell Senescence
|
| Published in |
Methods in molecular biology, January 2013
|
| DOI | 10.1007/978-1-62703-239-1_25 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-238-4, 978-1-62703-239-1
|
| Authors |
Narendra Wajapeyee, Sara K. Deibler, Michael R. Green, Wajapeyee, Narendra, Deibler, Sara K., Green, Michael R. |
| Abstract |
RNA interference (RNAi) is a powerful research tool that can be used to turn off-or silence-the expression of a specific gene. In recent years, RNAi screening on a genome-wide scale has provided the opportunity to identify factors and pathways involved in complex biological processes in a systematic and unbiased manner. Here we describe a genome-wide RNAi screening strategy to identify genes that are required for a particular type of cellular senescence called oncogene-induced senescence. The approach we describe is a general screening strategy that can be applied to the study of other forms of cellular senescence, including replicative senescence. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 13% |
| Unknown | 7 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 38% |
| Student > Ph. D. Student | 2 | 25% |
| Professor | 1 | 13% |
| Student > Postgraduate | 1 | 13% |
| Unknown | 1 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 38% |
| Agricultural and Biological Sciences | 3 | 38% |
| Chemistry | 1 | 13% |
| Unknown | 1 | 13% |