Chapter title |
Development and Characterization of Cell-Specific Androgen Receptor Knockout Mice
|
---|---|
Chapter number | 14 |
Book title |
The Nuclear Receptor Superfamily
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3724-0_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3722-6, 978-1-4939-3724-0
|
Authors |
Laura O’Hara, Lee B. Smith, O’Hara, Laura, Smith, Lee B. |
Abstract |
Conditional gene targeting has revolutionized molecular genetic analysis of nuclear receptor proteins, however development and analysis of such conditional knockouts is far from simple, with many caveats and pitfalls waiting to snare the novice or unprepared. In this chapter, we describe our experience of generating and analyzing mouse models with conditional ablation of the androgen receptor (AR) from tissues of the reproductive system and other organs. The guidance, suggestions, and protocols outlined in the chapter provide the key starting point for analyses of conditional-ARKO mice, completing them as described provides an excellent framework for further focussed project-specific analyses, and applies equally well to analysis of reproductive tissues from any mouse model generated through conditional gene targeting. |
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Mendeley readers
Geographical breakdown
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 38% |
Professor > Associate Professor | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Unknown | 3 | 38% |
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Unknown | 4 | 50% |