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Digital PCR

Overview of attention for book
Cover of 'Digital PCR'

Table of Contents

  1. Altmetric Badge
    Book Overview
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    Chapter 1 Entering the Pantheon of 21 st Century Molecular Biology Tools: A Perspective on Digital PCR
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    Chapter 2 Basic Concepts and Validation of Digital PCR Measurements
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    Chapter 3 Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies–What Does It Mean?
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    Chapter 4 Control Materials and Digital PCR Methods for Evaluation of Circulating Cell-Free DNA Extractions from Plasma
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    Chapter 5 Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize Events
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    Chapter 6 Using Droplet Digital PCR to Detect Coinfection of Human Herpesviruses 6A and 6B (HHV-6A and HHV-6B) in Clinical Samples
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    Chapter 7 Biomarkers in Cerebrospinal Fluid: Analysis of Cell-Free Circulating Mitochondrial DNA by Digital PCR
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    Chapter 8 Testing of General and Human-Associated Fecal Contamination in Waters
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    Chapter 9 Analyzing Copy Number Variation with Droplet Digital PCR
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    Chapter 10 Assessing HER2 Amplification in Plasma cfDNA
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    Chapter 11 Detection and Quantification of Mosaic Genomic DNA Variation in Primary Somatic Tissues Using ddPCR: Analysis of Mosaic Transposable-Element Insertions, Copy-Number Variants, and Single-Nucleotide Variants
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    Chapter 12 Monitoring of Response and Resistance in Plasma of EGFR -Mutant Lung Cancer Using Droplet Digital PCR
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    Chapter 13 Detection of Cancer DNA in Early Stage and Metastatic Breast Cancer Patients
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    Chapter 14 Droplet Digital PCR for Minimal Residual Disease Detection in Mature Lymphoproliferative Disorders
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    Chapter 15 Quantitation of JAK2 V617F Allele Burden by Using the QuantStudio™ 3D Digital PCR System
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    Chapter 16 Novel Multiplexing Strategies for Quantification of Rare Alleles Using ddPCR
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    Chapter 17 Identification and Use of Personalized Genomic Markers for Monitoring Circulating Tumor DNA
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    Chapter 18 Single Color Multiplexed ddPCR Copy Number Measurements and Single Nucleotide Variant Genotyping
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    Chapter 19 A Universal Droplet Digital PCR Approach for Monitoring of Graft Health After Transplantation Using a Preselected SNP Set
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    Chapter 20 Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci Using Droplet Digital PCR
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    Chapter 21 DNA Methylation Analysis Using Droplet Digital PCR
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    Chapter 22 Simultaneous Quantification of Multiple Alternatively Spliced mRNA Transcripts Using Droplet Digital PCR
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    Chapter 23 Using Droplet Digital PCR to Analyze Allele-Specific RNA Expression
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    Chapter 24 Very Low Abundance Single-Cell Transcript Quantification with 5-Plex ddPCRTM Assays
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    Chapter 25 Quantification of Circulating MicroRNAs by Droplet Digital PCR
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    Chapter 26 Droplet Digital PCR for Absolute Quantification of Extracellular MicroRNAs in Plasma and Serum: Quantification of the Cancer Biomarker hsa-miR-141
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    Chapter 27 Droplet Digital™ PCR Next-Generation Sequencing Library QC Assay
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    Chapter 28 Phasing DNA Markers Using Digital PCR
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    Chapter 29 ddTRAP: A Method for Sensitive and Precise Quantification of Telomerase Activity
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    Chapter 30 Highly Efficient and Reliable DNA Aptamer Selection Using the Partitioning Capabilities of ddPCR: The Hi-Fi SELEX Method
Attention for Chapter 9: Analyzing Copy Number Variation with Droplet Digital PCR
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (85th percentile)
  • High Attention Score compared to outputs of the same age and source (97th percentile)

Mentioned by

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1 blog
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1 X user
wikipedia
1 Wikipedia page

Citations

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15 Dimensions

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91 Mendeley
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Chapter title
Analyzing Copy Number Variation with Droplet Digital PCR
Chapter number 9
Book title
Digital PCR
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7778-9_9
Pubmed ID
29717442
Book ISBNs
978-1-4939-7776-5, 978-1-4939-7778-9
Authors

Avery Davis Bell, Christina L. Usher, Steven A. McCarroll, Bell, Avery Davis, Usher, Christina L., McCarroll, Steven A.

Abstract

Many genomic segments vary in copy number among individuals of the same species, or between cancer and normal cells within the same person. Correctly measuring this copy number variation is critical for studying its genetic properties, its distribution in populations and its relationship to phenotypes. Droplet digital PCR (ddPCR) enables accurate measurement of copy number by partitioning a PCR reaction into thousands of nanoliter-scale droplets, so that a genomic sequence of interest-whose presence or absence in a droplet is determined by end-point fluorescence-can be digitally counted. Here, we describe how we analyze copy number variants using ddPCR and review the design of effective assays, the performance of ddPCR with those assays, the optimization of reactions, and the interpretation of data.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 91 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 15 16%
Student > Ph. D. Student 14 15%
Student > Master 8 9%
Student > Bachelor 7 8%
Other 7 8%
Other 12 13%
Unknown 28 31%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 30 33%
Agricultural and Biological Sciences 11 12%
Medicine and Dentistry 6 7%
Neuroscience 2 2%
Computer Science 2 2%
Other 7 8%
Unknown 33 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 April 2022.
All research outputs
#2,791,460
of 23,047,237 outputs
Outputs from Methods in molecular biology
#532
of 13,196 outputs
Outputs of similar age
#65,010
of 442,433 outputs
Outputs of similar age from Methods in molecular biology
#34
of 1,499 outputs
Altmetric has tracked 23,047,237 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,196 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,433 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 1,499 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.

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