Chapter title |
Methods to Characterize Folding and Function of BamA Cross-Link Mutants
|
---|---|
Chapter number | 10 |
Book title |
The BAM Complex
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2871-2_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2870-5, 978-1-4939-2871-2
|
Authors |
Adam J. Kuszak, Nicholas Noinaj, Susan K. Buchanan, Kuszak, Adam J., Noinaj, Nicholas, Buchanan, Susan K. |
Abstract |
The utility of protein engineering, both the mutation and deletion of specific amino acids, to investigate protein structure and function has been demonstrated time and time again, and intermolecular and intramolecular interactions within the BAM complex and its individual components are no exception. Extensive efforts have probed conserved and unique amino acid sequences of the Bam proteins to define their functional roles. This chapter summarizes efforts as applied to the disulfide cross-link mutants of BamA and describes experimental methods used in our studies to determine that lateral opening of the barrel domain is required for function. |
Mendeley readers
Geographical breakdown
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Unknown | 3 | 100% |
Demographic breakdown
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Professor | 1 | 33% |
Student > Ph. D. Student | 1 | 33% |
Researcher | 1 | 33% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Agricultural and Biological Sciences | 1 | 33% |
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