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Mendeley readers
Chapter title |
The Role of a Destabilized Membrane for OMP Insertion
|
---|---|
Chapter number | 5 |
Book title |
The BAM Complex
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2871-2_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2870-5, 978-1-4939-2871-2
|
Authors |
Ashlee M. Plummer, Dennis Gessmann, Karen G. Fleming, Plummer, Ashlee M., Gessmann, Dennis, Fleming, Karen G. |
Abstract |
Here we describe the procedures used in our laboratory for the in vitro investigation of the apparent folding kinetics as well as the folding efficiencies of outer membrane proteins (OMPs). Because microbial OMPs display a change in their gel migration upon folding, the usage of traditional gel electrophoresis is a standard method of folding analysis. Additional aspects of the method we detail herein include the preparation and storage of OMP stocks, the setup procedures for a folding reaction, and the analysis of fraction folded from scanned gel images. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 33% |
Researcher | 1 | 17% |
Student > Doctoral Student | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 33% |
Agricultural and Biological Sciences | 1 | 17% |
Earth and Planetary Sciences | 1 | 17% |
Unknown | 2 | 33% |