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Enzyme Engineering

Overview of attention for book
Cover of 'Enzyme Engineering'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 A Tripartite Fusion System for the Selection of Protein Variants with Increased Stability In Vivo
  3. Altmetric Badge
    Chapter 2 Determining Enzyme Kinetics via Isothermal Titration Calorimetry
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    Chapter 3 GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria.
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    Chapter 4 Flow Cytometric Assays for Interrogating LAGLIDADG Homing Endonuclease DNA-Binding and Cleavage Properties
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    Chapter 5 TAL Effector Nuclease (TALEN) Engineering.
  7. Altmetric Badge
    Chapter 6 In Vitro Evolution of Enzymes
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    Chapter 7 Residue-Specific Incorporation of Unnatural Amino Acids into Proteins In Vitro and In Vivo
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    Chapter 8 Reconstructing Evolutionary Adaptive Paths for Protein Engineering
  10. Altmetric Badge
    Chapter 9 Oligonucleotide Recombination Enabled Site-Specific Mutagenesis in Bacteria
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    Chapter 10 FX Cloning: A Versatile High-Throughput Cloning System for Characterization of Enzyme Variants
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    Chapter 11 Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes
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    Chapter 12 Gene Synthesis by Assembly of Deoxyuridine-Containing Oligonucleotides
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    Chapter 13 Protein Engineering: Single or Multiple Site-Directed Mutagenesis
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    Chapter 14 Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative Recombination.
  16. Altmetric Badge
    Chapter 15 Promiscuity-Based Enzyme Selection for Rational Directed Evolution Experiments
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    Chapter 16 Rational Protein Sequence Diversification by Multi-Codon Scanning Mutagenesis
  18. Altmetric Badge
    Chapter 17 Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a Reporter
  19. Altmetric Badge
    Chapter 18 In Vitro Directed Evolution of Enzymes Expressed by E. coli in Microtiter Plates
Attention for Chapter 5: TAL Effector Nuclease (TALEN) Engineering.
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (75th percentile)
  • High Attention Score compared to outputs of the same age and source (82nd percentile)

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Chapter title
TAL Effector Nuclease (TALEN) Engineering.
Chapter number 5
Book title
Enzyme Engineering
Published in
Methods in molecular biology, February 2013
DOI 10.1007/978-1-62703-293-3_5
Pubmed ID
Book ISBNs
978-1-62703-292-6, 978-1-62703-293-3
Authors

Li T, Yang B, Ting Li, Bing Yang, Li, Ting, Yang, Bing

Abstract

TALENs, fusion proteins of DNA binding domains of TAL (transcription activator-like) effectors and the DNA cleavage domains of endonuclease FokI, have emerged as genetic tools for targeted gene modification, holding great potential for basic and applied research, even for gene therapy. Here we present a simple and efficient approach to custom-engineering TALEN genes with four basic TAL repeats and their DNA recognition cipher. The "modular assembly" method also involves the "Golden Gate" cloning strategy, using 53 ready-to-use plasmids in just two rounds of restriction and ligation to assemble TALENs with up to 24 repeat units that recognize up to 24 bp of target DNA.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Unknown 53 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 14 26%
Student > Ph. D. Student 11 20%
Student > Bachelor 6 11%
Student > Master 5 9%
Professor > Associate Professor 4 7%
Other 9 17%
Unknown 5 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 26 48%
Biochemistry, Genetics and Molecular Biology 15 28%
Medicine and Dentistry 3 6%
Immunology and Microbiology 2 4%
Materials Science 1 2%
Other 0 0%
Unknown 7 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 March 2015.
All research outputs
#5,876,115
of 22,796,179 outputs
Outputs from Methods in molecular biology
#1,710
of 13,111 outputs
Outputs of similar age
#47,992
of 193,108 outputs
Outputs of similar age from Methods in molecular biology
#7
of 39 outputs
Altmetric has tracked 22,796,179 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 13,111 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 193,108 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 39 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.