Chapter title |
TAL Effector Nuclease (TALEN) Engineering.
|
---|---|
Chapter number | 5 |
Book title |
Enzyme Engineering
|
Published in |
Methods in molecular biology, February 2013
|
DOI | 10.1007/978-1-62703-293-3_5 |
Pubmed ID | |
Book ISBNs |
978-1-62703-292-6, 978-1-62703-293-3
|
Authors |
Li T, Yang B, Ting Li, Bing Yang, Li, Ting, Yang, Bing |
Abstract |
TALENs, fusion proteins of DNA binding domains of TAL (transcription activator-like) effectors and the DNA cleavage domains of endonuclease FokI, have emerged as genetic tools for targeted gene modification, holding great potential for basic and applied research, even for gene therapy. Here we present a simple and efficient approach to custom-engineering TALEN genes with four basic TAL repeats and their DNA recognition cipher. The "modular assembly" method also involves the "Golden Gate" cloning strategy, using 53 ready-to-use plasmids in just two rounds of restriction and ligation to assemble TALENs with up to 24 repeat units that recognize up to 24 bp of target DNA. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 2% |
Unknown | 53 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 14 | 26% |
Student > Ph. D. Student | 11 | 20% |
Student > Bachelor | 6 | 11% |
Student > Master | 5 | 9% |
Professor > Associate Professor | 4 | 7% |
Other | 9 | 17% |
Unknown | 5 | 9% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 26 | 48% |
Biochemistry, Genetics and Molecular Biology | 15 | 28% |
Medicine and Dentistry | 3 | 6% |
Immunology and Microbiology | 2 | 4% |
Materials Science | 1 | 2% |
Other | 0 | 0% |
Unknown | 7 | 13% |