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Mendeley readers
Chapter title |
Spatial Organization of Enzyme Cascade on a DNA Origami Nanostructure
|
---|---|
Chapter number | 11 |
Book title |
3D DNA Nanostructure
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6454-3_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6452-9, 978-1-4939-6454-3
|
Authors |
Jinglin Fu, Tianran Li, Fu, Jinglin, Li, Tianran |
Abstract |
Self-assembled DNA nanostructures hold great promise to organize multi-enzyme systems with the precise control of the geometric arrangements. Enzymes modified with single-stranded DNA anchors are assembled onto the DNA origami tiles by hybridizing with the corresponding complementary strands displayed on the surface of the DNA nanostructures. Here, we describe a protocol of assembling a two-enzyme cascade on a discrete, rectangular DNA origami tile, where the distance between enzymes is precisely controlled for investigating the distance-dependent cascade activities. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 25% |
Student > Doctoral Student | 2 | 25% |
Other | 1 | 13% |
Student > Ph. D. Student | 1 | 13% |
Student > Bachelor | 1 | 13% |
Other | 0 | 0% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 38% |
Agricultural and Biological Sciences | 1 | 13% |
Physics and Astronomy | 1 | 13% |
Medicine and Dentistry | 1 | 13% |
Chemistry | 1 | 13% |
Other | 0 | 0% |
Unknown | 1 | 13% |