Chapter title |
DNA G-Quadruplex-Based Assay of Enzyme Activity
|
---|---|
Chapter number | 10 |
Book title |
3D DNA Nanostructure
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6454-3_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6452-9, 978-1-4939-6454-3
|
Authors |
Zhuoliang Liu, Kaiyu He, Wang Li, Xin Liu, Xiahong Xu, Zhou Nie, Shouzhuo Yao, Liu, Zhuoliang, He, Kaiyu, Li, Wang, Liu, Xin, Xu, Xiahong, Nie, Zhou, Yao, Shouzhuo |
Abstract |
DNA G-quadruplexes are special three-dimensional (3D) DNA nanostructures formed by specific G-rich DNA sequences. These 3D DNA nanostructures can bind with hemin and significantly improve the intrinsic peroxidase activity of hemin. Besides this function, they also enhance the fluorescence intensity of some G-quadruplex-specific dyes. Owing to these features, G-quadruplexes possess several superiorities in the detection of enzymes involved in nucleic acid metabolism, including facile probe fabrication without labeling, simple detection process without washing or separation steps, rapid observation by naked eyes, and easy integration with nucleic acid amplification strategies to amplify signals. Herein, we describe two strategies for label-free detection of enzyme activity based on DNA G-quadruplexes. To increase sensitivity, template-dependent and template-independent DNA amplifications were introduced for the amplification of G-rich DNA sequences. DNA methyltransferase and terminal deoxynucleotidyl transferase were detected as two model analytes, respectively. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 29% |
Student > Master | 1 | 14% |
Unknown | 4 | 57% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 1 | 14% |
Unknown | 6 | 86% |