Chapter title |
Designing DNA Nanotube Liquid Crystals as a Weak-Alignment Medium for NMR Structure Determination of Membrane Proteins
|
---|---|
Chapter number | 14 |
Book title |
3D DNA Nanostructure
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6454-3_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6452-9, 978-1-4939-6454-3
|
Authors |
John Min, William M. Shih, Gaëtan Bellot, Min, John, Shih, William M., Bellot, Gaëtan |
Abstract |
Thirty percent of the human proteome is composed of membrane proteins that can perform a wide range of cellular functions and communications. They represent the core of modern medicine as the targets of about 50 % of all prescription pharmaceuticals. However, elucidating the structure of membrane proteins has represented a constant challenge, even in the modern era. To date, only a few hundred high-resolution structural models of membrane proteins are available. This chapter describes the emergence of DNA nanotechnology as a powerful tool for the structural characterization of membrane protein using solution-state nuclear magnetic resonance (NMR) spectroscopy. Here, we detail the large-scale synthesis of detergent-resistant DNA nanotubes that can be assembled into a dilute liquid crystal to be used as a weak-alignment media in solution NMR structure determination of membrane proteins. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Unknown | 6 | 75% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 13% |
Immunology and Microbiology | 1 | 13% |
Unknown | 6 | 75% |