| Chapter title |
Cellular Model of p21-Induced Senescence
|
|---|---|
| Chapter number | 3 |
| Book title |
Oncogene-Induced Senescence
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6670-7_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6668-4, 978-1-4939-6670-7
|
| Authors |
Michael Shtutman, Bey-Dih Chang, Gary P. Schools, Eugenia V. Broude, Shtutman, Michael, Chang, Bey-Dih, Schools, Gary P., Broude, Eugenia V. |
| Abstract |
Cellular senescence is a unique process of normal physiology, from embryonic development to aging, also known for its association with a broad range of pathological conditions. Therefore a reliable model of cellular senescence remains an indispensable tool for the investigation of senescence-associated changes and human disease. Here we describe a model of HT1080 fibrosarcoma cells with an inducible senescence phenotype. These cells are equipped with the lac repressor and exogenous p21 under the control of a lac repressor regulated promoter. The senescent phenotype is induced in these cells by isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible expression of senescence-associated cell cycle inhibitor p21(Waf1/Cip1/Sdi1). |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 97 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 13 | 13% |
| Student > Ph. D. Student | 13 | 13% |
| Student > Bachelor | 11 | 11% |
| Researcher | 9 | 9% |
| Student > Doctoral Student | 3 | 3% |
| Other | 9 | 9% |
| Unknown | 39 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 30 | 31% |
| Medicine and Dentistry | 9 | 9% |
| Agricultural and Biological Sciences | 7 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 4% |
| Immunology and Microbiology | 3 | 3% |
| Other | 4 | 4% |
| Unknown | 40 | 41% |