Chapter title |
Detection of Dysfunctional Telomeres in Oncogene-Induced Senescence
|
---|---|
Chapter number | 6 |
Book title |
Oncogene-Induced Senescence
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6670-7_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6668-4, 978-1-4939-6670-7
|
Authors |
Priyanka L. Patel, Utz Herbig, Patel, Priyanka L., Herbig, Utz |
Abstract |
Expressing oncogenes in normal somatic human cells leads to cellular senescence after just a few cell division cycles. In cells that are more resistant to culture stresses, such as human dermal fibroblasts, this oncogene-induced senescence (OIS) is a result of a DNA damage response (DDR) that is activated due to the formation of DNA lesions at both non-telomeric and telomeric DNA sequences. DNA lesions can be visualized as DDR foci by immunofluorescence microscopy using antibodies against a number of DDR factors, including ϒ-H2AX and 53BP1. Over time and as cells remain arrested in OIS, non-telomeric DDR foci progressively become resolved, while telomeric DDR foci, also called dysfunctional telomeres, persist. Here we describe a protocol to detect dysfunctional telomeres in cultured human cells, to monitor a temporal enrichment of dysfunctional telomeres in cells that had undergone OIS, and to detect dysfunctional telomeres in paraffin-embedded and formalin-fixed human tissue. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 57% |
Researcher | 1 | 14% |
Student > Master | 1 | 14% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Agricultural and Biological Sciences | 2 | 29% |
Medicine and Dentistry | 2 | 29% |
Unknown | 1 | 14% |