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Eosinophils

Overview of attention for book
Cover of 'Eosinophils'

Table of Contents

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    Book Overview
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    Chapter 1 Eosinophil overview: structure, biological properties, and key functions.
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    Chapter 2 Eosinophil Purification from Peripheral Blood
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    Chapter 3 Eosinophil purification from human bone marrow.
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    Chapter 4 CD34+ Eosinophil-Lineage-Committed Cells in the Mouse Lung
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    Chapter 5 Eosinophil Cell Lines
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    Chapter 6 Cell Signalling During Human Eosinophil Differentiation
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    Chapter 7 Eosinophil Intracellular Signalling: Apoptosis
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    Chapter 8 Identification of Human Eosinophils in Whole Blood by Flow Cytometry
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    Chapter 9 Isolation and Functional Assessment of Eosinophil Crystalloid Granules
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    Chapter 10 Eosinophil Chemotaxis
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    Chapter 11 Eosinophil shape change and secretion.
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    Chapter 12 Human eosinophil adhesion and receptor expression.
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    Chapter 13 Eosinophils
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    Chapter 14 Human Eosinophil Transmigration
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    Chapter 15 Measurement of eosinophil kinetics in healthy volunteers.
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    Chapter 16 Assays of Eosinophil Apoptosis and Phagocytic Uptake
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    Chapter 17 Clinical Measurement of Eosinophil Numbers in Eosinophilic Conjunctivitis
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    Chapter 18 Qualitative and quantitative studies of eosinophils in parasitic infections.
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    Chapter 19 Interactions of Eosinophils with Nerves
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    Chapter 20 Eosinophils interaction with mast cells: the allergic effector unit.
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    Chapter 21 Eosinophil interactions: antigen presentation.
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    Chapter 22 Eosinophils and Respiratory Virus Infection: A Dual-Standard Curve qRT-PCR-Based Method for Determining Virus Recovery from Mouse Lung Tissue
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    Chapter 23 Antimicrobial activity of human eosinophil granule proteins.
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    Chapter 24 Eosinophils and the Ovalbumin Mouse Model of Asthma
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    Chapter 25 Mutant Mice and Animal Models of Airway Allergic Disease
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    Chapter 26 Murine Models of Eosinophilic Leukemia: A Model of FIP1L1-PDGFRα Initiated Chronic Eosinophilic Leukemia/Systemic Mastocytosis.
Attention for Chapter 11: Eosinophil shape change and secretion.
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Chapter title
Eosinophil shape change and secretion.
Chapter number 11
Book title
Eosinophils
Published in
Methods in molecular biology, July 2014
DOI 10.1007/978-1-4939-1016-8_11
Pubmed ID
24986612
Book ISBNs
978-1-4939-1015-1, 978-1-4939-1016-8
Authors

Willetts L, Ochkur SI, Jacobsen EA, Lee JJ, Lacy P, Lian Willetts, Sergei I. Ochkur, Elizabeth A. Jacobsen, James J. Lee, Paige Lacy, Willetts, Lian, Ochkur, Sergei I., Jacobsen, Elizabeth A., Lee, James J., Lacy, Paige

Abstract

The analysis of eosinophil shape change and mediator secretion is a useful tool in understanding how eosinophils respond to immunological stimuli and chemotactic factors. Eosinophils undergo dramatic shape changes, along with secretion of the granule-derived enzyme eosinophil peroxidase (EPX) in response to chemotactic stimuli including platelet-activating factor and CCL11 (eotaxin-1). Here, we describe the analysis of eosinophil shape change by confocal microscopy analysis and provide an experimental approach for comparing unstimulated cells with those that have been stimulated to undergo chemotaxis. In addition, we illustrate two different degranulation assays for EPX using OPD and an enzyme-linked immunosorbent assay technique and show how eosinophil degranulation may be assessed from in vitro as well as ex vivo stimulation.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 36%
Professor 3 21%
Student > Ph. D. Student 2 14%
Unknown 4 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 21%
Immunology and Microbiology 3 21%
Chemistry 1 7%
Medicine and Dentistry 1 7%
Unknown 6 43%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 February 2015.
All research outputs
#20,262,276
of 22,792,160 outputs
Outputs from Methods in molecular biology
#9,898
of 13,110 outputs
Outputs of similar age
#192,092
of 227,524 outputs
Outputs of similar age from Methods in molecular biology
#44
of 100 outputs
Altmetric has tracked 22,792,160 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,110 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 227,524 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 100 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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