Chapter title |
The structure of the mer operon.
|
---|---|
Chapter number | 49 |
Book title |
Plasmids in Bacteria
|
Published in |
Basic life sciences, January 1985
|
DOI | 10.1007/978-1-4613-2447-8_49 |
Pubmed ID | |
Book ISBNs |
978-1-4612-9487-0, 978-1-4613-2447-8
|
Authors |
Patricia Barrineau, Pearce Gilbert, W. James Jackson, Cheryl S. Jones, Anne O. Summers, Sonya Wisdom, Barrineau, Patricia, Gilbert, Pearce, Jackson, W. James, Jones, Cheryl S., Summers, Anne O., Wisdom, Sonya |
Abstract |
The DNA sequence has been determined for a 3.8-kb region which encodes the mercury-resistance (mer) operon of the IncFII plasmid NR1. The sequence reveals 4 open reading frames which could encode proteins of 12,522, 9,429, 14,965, and 58,912 d corresponding to the 4 previously described Hg-inducible proteins detected in minicells carrying mer+ plasmids. The Hg(II) reductase protein sequence is about 90% homologous to that of Tn501, but the DNA sequence shows a homology of 60-70% to that of Tn501 except for short regions of very high homology. The entire mer region is 63.4% G-C overall. The region encoding the merR (positive regulatory) function has 3 possible open reading frames, 2 of which overlap in one direction and the third of which reads in the opposite direction. Attempts to visualize the polypeptide(s) encoded by the merR cistron were unsuccessful. |
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