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Plasmids in Bacteria

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Cover of 'Plasmids in Bacteria'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Introduction
  3. Altmetric Badge
    Chapter 2 Plasmids as Organisms
  4. Altmetric Badge
    Chapter 3 Report on a Workshop: Structure and Function
  5. Altmetric Badge
    Chapter 4 Evolutionary Relevance of Genetic Rearrangements Involving Plasmids
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    Chapter 5 Mechanisms of transposition in bacteria.
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    Chapter 6 Insertion of Transcriptional Elements Outside the Replication Region can Interfere with Replication, Maintenance, and Stability of ColE1-Derived Plasmids
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    Chapter 7 Studies on the Transposition of IS1
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    Chapter 8 On the Transposition and Evolution of Tn 1721 and its Relatives
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    Chapter 9 Repeated DNA Sequences Recombine 1,000 Times More Frequently in a Plasmid Than in the Chromosome of Bacillus Subtilis
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    Chapter 10 Mercury Resistance Transposon Tn 813 Mediates Chromosome Transfer in Rhodopseudomonas Sphaeroides and Intergeneric Transfer of pBR322
  12. Altmetric Badge
    Chapter 11 Report on a Workshop: Plasmids in Unusual Systems
  13. Altmetric Badge
    Chapter 12 Chairman’s Introduction: Replication, Incompatibility, and Partition
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    Chapter 13 Role of the π Initiation Protein and Direct Nucleotide Sequence Repeats in the Regulation of Plasmid R6K Replication
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    Chapter 14 Initiation of replication of the Escherichia coli chromosomal origin reconstituted with purified enzymes.
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    Chapter 15 Origin and Initiation Sites of λdv DNA Replication In Vitro
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    Chapter 16 Broad Host-Range Plasmid R1162: Replication, Incompatibility, and Copy-Number Control
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    Chapter 17 Control of Plasmid Replication: Theoretical Considerations and Practical Solutions
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    Chapter 18 The Partition Functions of P1, P7, and F Miniplasmids
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    Chapter 19 Genetic Interactions of Broad Host-Range Plasmid RK2: Evidence for a Complex Replication Regulon
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    Chapter 20 Replication Determinants of the Broad Host-Range Plasmid RSF1010
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    Chapter 21 Regulation of Replication and Maintenance Functions of Broad Host-Range Plasmid RK2
  23. Altmetric Badge
    Chapter 22 Control of Chromosome Replication in Bacteria
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    Chapter 23 Stable Maintenance of Plasmid CLO DF13: Structural and Functional Relationships Between Replication Control, Partitioning, and Incompatibility
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    Chapter 24 Replication control for pT181, an indirectly regulated plasmid.
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    Chapter 25 Construction of ColE1 RNA1 Mutants and Analysis of their Function In Vivo
  27. Altmetric Badge
    Chapter 26 Incompatibility and IncFII plasmid replication control.
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    Chapter 27 P1 Plasmid Maintenance: A Paradigm of Precise Control
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    Chapter 28 Partitioning of the pSC101 plasmid during cell division.
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    Chapter 29 DNA-protein interaction at the replication origins of plasmid chromosomes.
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    Chapter 30 Positive Regulation and Transcription Initiation of XYL Operons on TOL Plasmid
  32. Altmetric Badge
    Chapter 31 Chairmen’s Introduction: Plasmid Transfer
  33. Altmetric Badge
    Chapter 32 Linear Plasmids with Terminal Inverted Repeats Obtained from Streptomyces Rochei and Kluyveromyces Lactis
  34. Altmetric Badge
    Chapter 33 Conjugal Plasmid Transfer in Bacillus Thuringiensis
  35. Altmetric Badge
    Chapter 34 Mechanisms Essential for Stable Inheritance of Mini-F Plasmid
  36. Altmetric Badge
    Chapter 35 Sex Pheromones and Plasmid Transfer in Streptococcus Faecalis : A Pheromone, cAM373, Which is Also Excreted by Staphylococcus Aureus
  37. Altmetric Badge
    Chapter 36 General Genetic Recombination of Bacterial Plasmids
  38. Altmetric Badge
    Chapter 37 The Origin of Plasmid DNA Transfer During Bacterial Conjugation
  39. Altmetric Badge
    Chapter 38 Genes and gene products involved in the synthesis of F-pili.
  40. Altmetric Badge
    Chapter 39 Genetics of Clindamycin Resistance in Bacteroides
  41. Altmetric Badge
    Chapter 40 Effect of Chromosome Homology of Plasmid Transformation and Plasmid Conjugal Transfer in Haemophilus Influenzae
  42. Altmetric Badge
    Chapter 41 Plasmid DNA Primases and their Role in Bacterial Conjugation
  43. Altmetric Badge
    Chapter 42 Promoters in the Transfer Region of Plasmid F
  44. Altmetric Badge
    Chapter 43 Conjugative Sex Plasmids of Streptomyces
  45. Altmetric Badge
    Chapter 44 The Structure and Source of Plasmid DNA Determine the Cloning Properties of Vectors for Bacillus Subtilis
  46. Altmetric Badge
    Chapter 45 Shuttle Vector for Escherichia Coli , Pseudomonas Putida , and Pseudomonas Aeruginosa
  47. Altmetric Badge
    Chapter 46 Report on a Workshop: Plasmid Cloning Vehicles
  48. Altmetric Badge
    Chapter 47 Plasmids in the Degradation of Chlorinated Aromatic Compounds
  49. Altmetric Badge
    Chapter 48 Notes on Metabolic Plasmid Organization and Expression
  50. Altmetric Badge
    Chapter 49 The structure of the mer operon.
  51. Altmetric Badge
    Chapter 50 Analysis and Manipulation of Plasmid-Encoded Pathways for the Catabolism of Aromatic Compounds by Soil Bacteria
  52. Altmetric Badge
    Chapter 51 The Plasmid-Specified Aerobactin Iron Uptake System of Escherichia Coli
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    Chapter 52 Plasmid-Mediated Iron Sequestering Systems in Pathogenic Strains of Vibrio Anguillarum and Escherichia Coli
  54. Altmetric Badge
    Chapter 53 Chairman’s Introduction: Specialized Functions
  55. Altmetric Badge
    Chapter 54 Cloning of an Enzymatically Active Segment of the Exotoxin-A Gene of Pseudomonas Aeruginosa
  56. Altmetric Badge
    Chapter 55 Structure, Function, and Regulation of the Plasmid-Encoded Hemolysin Determinant of Escherichia Coli
  57. Altmetric Badge
    Chapter 56 pIAA, A Virulence Plasmid in Pseudomonas Savastanoi
  58. Altmetric Badge
    Chapter 57 The molecular basis of plant cell transformation by Agrobacterium tumefaciens.
  59. Altmetric Badge
    Chapter 58 Post-Transcriptional Regulation of Chloramphenicol Acetyl Transferase
  60. Altmetric Badge
    Chapter 59 Plasmid and Larval Toxin of Bacillus Laterosporus
  61. Altmetric Badge
    Chapter 60 Replication, Incompatibility, Partition
  62. Altmetric Badge
    Chapter 61 Specialized Functions
  63. Altmetric Badge
    Chapter 62 Structure and Evolution
  64. Altmetric Badge
    Chapter 63 Transfer
  65. Altmetric Badge
    Chapter 64 Vectors
  66. Altmetric Badge
    Chapter 65 Speakers
  67. Altmetric Badge
    Chapter 66 Participants
Attention for Chapter 24: Replication control for pT181, an indirectly regulated plasmid.
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Chapter title
Replication control for pT181, an indirectly regulated plasmid.
Chapter number 24
Book title
Plasmids in Bacteria
Published in
Basic life sciences, January 1985
DOI 10.1007/978-1-4613-2447-8_24
Pubmed ID
Book ISBNs
978-1-4612-9487-0, 978-1-4613-2447-8
Authors

Richard P. Novick, Steven J. Projan, C. Chandra Kumar, Stephen Carleton, Alexandra Gruss, Sarah K. Highlander, John Kornblum, Novick, Richard P., Projan, Steven J., Kumar, C. Chandra, Carleton, Stephen, Gruss, Alexandra, Highlander, Sarah K., Kornblum, John

Abstract

PT181 is a fully sequenced Staphylococcus aureus plasmid whose size is 4,437 bp. It specifies tetracycline resistance and has a copy number of about 22 per cell in exponentially growing cultures. The functional organization of the pT181 replicon is centered around the coding sequence for a 35-kd protein, RepC, that is absolutely required for replication of the plasmid. The replication origin is contained within the repC coding sequence and the region immediately 5' to the RepC start is involved in control of the plasmid replication rate. PT181 replication is controlled at the level of RepC synthesis by a negative regulatory system that is functionally similar to that of the Co1E1 and IncFII plasmids of Escherichia coli. The pT181 control circuit involves 2 short transcripts, RNA I and RNA II, that are transcribed from the region specifying the 5' end of the untranslated repC mRNA leader and in the opposite direction. These are referred to as countertranscripts. The countertranscripts regulate RepC synthesis by a mechanism that probably involves interaction with the repC mRNA leader in a manner that interferes with translation. Both of the countertranscripts seem to be necessary for normal replication control; their separate roles remain unclear. Unlike plasmids of the Co1E1 and IncFII groups, plasmids such as Co1E1 are considered to have direct regulation of replication because the inhibitory element of the copy control circuit directly inhibits the initiation of replication. Plasmids such as pT181 are considered to have indirect regulation of replication because the product of the regulated step, RepC, is trans-active. Plasmids of the IncFII type are considered to have direct regulation of replication because the product of the regulated step, RepA is cis-active The analysis of pT181 replication physiology has illustrated 2 important differences between directly and indirectly regulated plasmids: a) for directly regulated plasmids, copy mutants specifying a normal inhibitor substance but an inactive target site exclude the wild-type or recessive mutants by directly interfering with their replication. Analogous mutants of indirectly regulated plasmids coexist readily with the wild-type and all mutants (although they do manifest segregational incompatibility) because the Rep protein is always shared by all plasmids in the cell, regardless of its source. b) Mutations of directly regulated plasmids in the region where target transcript and countertranscript overlap may give rise to totally new incompatibility groups because they engender independently self-correcting copy pools.(ABSTRACT TRUNCATED AT 400 WORDS)

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The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 13%
Unknown 7 88%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 50%
Professor 1 13%
Other 1 13%
Unknown 2 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 25%
Immunology and Microbiology 2 25%
Biochemistry, Genetics and Molecular Biology 1 13%
Medicine and Dentistry 1 13%
Unknown 2 25%