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Mendeley readers
Attention Score in Context
| Chapter title |
Enzymatic Modification of 5′-Capped RNA and Subsequent Labeling by Click Chemistry
|
|---|---|
| Chapter number | 3 |
| Book title |
Synthetic mRNA
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3625-0_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3623-6, 978-1-4939-3625-0
|
| Authors |
Josephin M. Holstein, Daniela Stummer, Andrea Rentmeister, Holstein, Josephin M., Stummer, Daniela, Rentmeister, Andrea |
| Abstract |
The combination of enzymatic modification and bioorthogonal click chemistry provides a powerful approach for site-specific labeling of different classes of biomolecules in vitro and even in cellular environments. Herein, we describe a chemoenzymatic method to site specifically label 5'-capped model mRNAs independent of their sequence. A trimethylguanosine synthase was engineered to introduce alkyne, azido, or 4-vinylbenzyl moieties to the 5'-cap. These functional groups were then used for labeling using typical click reactions, such as the azide-alkyne cycloaddition or the tetrazine ligation. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 30% |
| Student > Bachelor | 2 | 20% |
| Unknown | 5 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 30% |
| Chemistry | 3 | 30% |
| Unknown | 4 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 December 2023.
All research outputs
#8,446,639
of 25,223,158 outputs
Outputs from Methods in molecular biology
#2,618
of 14,138 outputs
Outputs of similar age
#128,910
of 405,901 outputs
Outputs of similar age from Methods in molecular biology
#277
of 1,463 outputs
Altmetric has tracked 25,223,158 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 14,138 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 405,901 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 1,463 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.