| Chapter title |
Assessing DNA Structures with 125 I Radioprobing
|
|---|---|
| Chapter number | 9 |
| Book title |
G-Quadruplex DNA
|
| Published in |
Methods in molecular biology, January 2010
|
| DOI | 10.1007/978-1-59745-363-9_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-58829-950-5, 978-1-59745-363-9
|
| Authors |
Timur I. Gaynutdinov, Ronald D. Neumann, Igor G. Panyutin, Gaynutdinov, Timur I., Neumann, Ronald D., Panyutin, Igor G. |
| Abstract |
Iodine-125 radioprobing is based on incorporation of radioiodine into a defined position in a nucleic acid molecule. Decay of (125)I results in the emission of multiple, low-energy Auger electrons that, along with positively charged residual daughter nuclide, produce DNA strand breaks. The probability of such strand breaks at a given nucleotide is in inverse proportion to the distance from the (125)I atom to the sugar of that nucleotide. Therefore, conclusions can be drawn about the conformation or folding of a DNA or RNA molecule based on the distribution of (125)I decay-induced strand breaks. Here we describe in detail the application (125)I radioprobing for studying the conformation of quadruplex structures, and discuss the advantages and limitations of the method. |
Mendeley readers
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|---|---|---|
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| Unknown | 3 | 25% |