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Chapter title |
Expression of normal and variant human hypoxanthine-guanine phosphoribosyltransferase in E. coli.
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Chapter number | 23 |
Book title |
Purine and Pyrimidine Metabolism in Man VII
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Published in |
Advances in experimental medicine and biology, January 1991
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DOI | 10.1007/978-1-4615-7703-4_23 |
Pubmed ID | |
Book ISBNs |
978-1-4615-7705-8, 978-1-4615-7703-4
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Authors |
Davidson, B L, Roessler, B J, Palella, T D, Davidson, Beverly L., Roessler, Blake J., Palella, Thomas D., Beverly L. Davidson, Blake J. Roessler, Thomas D. Palella |
Abstract |
1. ECPR is a rapid and effective means for generating recombinant human HPRT. 2. The Bl21 (DE3) T7 polymerase/T7 promoter system provides high level expression of human HPRT constructs after induction of the T7 polymerase gene with IPTG. 3. Human HPRT constructs expressed in E. coli mimic the variant properties originally demonstrated in lymphoblast extracts from affected individuals. 4. Human HPRT expressed in E. coli can be rapidly purified to near homogeneity by a two step purification scheme. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 33% |
Researcher | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 33% |
Medicine and Dentistry | 1 | 33% |
Unknown | 1 | 33% |