Chapter title |
Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells
|
---|---|
Chapter number | 8 |
Book title |
Plant Vacuolar Trafficking
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7856-4_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7855-7, 978-1-4939-7856-4
|
Authors |
Jorge M. P. Rodrigues, Cátia S. Pereira, Natacha Fontes, Hernâni Gerós, Manuela Côrte-Real, Rodrigues, Jorge M. P., Pereira, Cátia S., Fontes, Natacha, Gerós, Hernâni, Côrte-Real, Manuela |
Abstract |
A series of optimized protocols to isolate vacuoles from both yeast and plant cells, and to characterize the purified organelles at a functional and structural level, are described. For this purpose, we took advantage of the combined use of cell fractionation techniques with different fluorescence-based approaches namely flow cytometry, fluorescence microscopy and spectrofluorimetry. These protocols altogether constitute valuable tools for the study of vacuole structure and function, as well as for the high-throughput screening of drug libraries to identify new molecules that target the vacuole. |
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