| Chapter title |
HILIC-MS/MS Multi-Targeted Method for Metabolomics Applications
|
|---|---|
| Chapter number | 5 |
| Book title |
Metabolic Profiling
|
| Published in |
Methods in molecular biology, April 2018
|
| DOI | 10.1007/978-1-4939-7643-0_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7642-3, 978-1-4939-7643-0
|
| Authors |
Christina Virgiliou, Helen G. Gika, Georgios A. Theodoridis, Virgiliou, Christina, Gika, Helen G., Theodoridis, Georgios A. |
| Abstract |
Metabolomics aims at the identification and quantification of key-end point metabolites, basically polar, in order to study changes in biochemical activities in response to pathophysiological stimuli or genetic modifications. Targeted profiling assays have enjoyed a growing popularity during the last years with LC-MS/MS as a powerful tool for development of such (semi-) quantitative methods for a large number of metabolites. Here we describe a method for absolute quantification of ca. 100 metabolites belonging to key metabolite classes such as sugars, amino acids, nucleotides, organic acids, and amines with a hydrophilic interaction liquid chromatography (HILIC) system comprised of ultra (high) performance liquid chromatography (UHPLC) with detection on a triple-quadrupole mass spectrometer operating in both positive and negative electrospray ionization modes. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 23% |
| Student > Bachelor | 3 | 14% |
| Researcher | 2 | 9% |
| Student > Master | 2 | 9% |
| Professor | 1 | 5% |
| Other | 3 | 14% |
| Unknown | 6 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 5 | 23% |
| Agricultural and Biological Sciences | 4 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 14% |
| Biochemistry, Genetics and Molecular Biology | 3 | 14% |
| Medicine and Dentistry | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 5 | 23% |