Chapter title |
Assessment of airway hyperresponsiveness in murine tracheal rings.
|
---|---|
Chapter number | 20 |
Book title |
Mouse Models of Allergic Disease
|
Published in |
Methods in molecular biology, August 2013
|
DOI | 10.1007/978-1-62703-496-8_20 |
Pubmed ID | |
Book ISBNs |
978-1-62703-495-1, 978-1-62703-496-8
|
Authors |
Herlihy JT, Semenov I, Brenner R, Jeremiah T. Herlihy, Iurii Semenov, Robert Brenner, Herlihy, Jeremiah T., Semenov, Iurii, Brenner, Robert |
Abstract |
Isolated tracheal rings have often been used to directly measure the contractile output of airway smooth muscle (ASM). Here, we describe the method for excising murine tracheas, mounting tracheal rings in organ baths, and measuring the isometric forces generated by the ASM when stimulated by drug additions or electric field stimulation. The apparatus for the setup and the pathways responsible for stimulation are detailed. Examples of the responses and analyses of two types of ASM stimulation are illustrated: (1) the carbachol concentration-response curve and (2) the frequency-response curve elicited by electric field stimulation. |
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