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Proteomics

Overview of attention for book
Cover of 'Proteomics'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Introduction to Proteomics
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    Chapter 2 High-Resolution Two-Dimensional Electrophoresis
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    Chapter 3 Non-classical 2-D Electrophoresis
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    Chapter 4 Proteomics
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    Chapter 5 MALDI MS
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    Chapter 6 Capillary Electrophoresis Coupled to Mass Spectrometry for Proteomic Profiling of Human Urine and Biomarker Discovery
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    Chapter 7 A Newcomer’s Guide to Nano-Liquid-Chromatography of Peptides
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    Chapter 8 Multidimensional Protein Identification Technology
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    Chapter 9 Characterization of Platelet Proteins Using Peptide Centric Proteomics
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    Chapter 10 Proteomics
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    Chapter 11 Liquid Chromatography–Mass Spectrometry-Based Quantitative Proteomics
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    Chapter 12 iTRAQ-Labeling of In-Gel Digested Proteins for Relative Quantification
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    Chapter 13 Electrospray Mass Spectrometry for Quantitative Plasma Proteome Analysis
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    Chapter 14 Algorithms and Databases
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    Chapter 15 Shotgun Protein Identification and Quantification by Mass Spectrometry
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    Chapter 16 Proteomics Identification of Oxidatively Modified Proteins in Bra
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    Chapter 17 Isotope-Labeling and Affinity Enrichment of Phosphopeptides for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 18 Part VII Subcellular Proteomics Organelle Proteomics: Reduction of Sample Complexity by Enzymatic In-Gel Selection of Native Proteins
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    Chapter 19 Isolation of Plasma Membranes from the Nervous System by Countercurrent Distribution in Aqueous Polymer Two-Phase Systems
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    Chapter 20 Enrichment and Preparation of Plasma Membrane Proteins from Arabidopsis thaliana for Global Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 21 Tandem Affinity Purification of Protein Complexes from Mammalian Cells by the Strep/FLAG (SF)-TAP Tag
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    Chapter 22 Sequential Peptide Affinity Purification System for the Systematic Isolation and Identification of Protein Complexes from Escherichia coli
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    Chapter 23 Bioinformatical Approaches to Detect and Analyze Protein Interactions
Attention for Chapter 6: Capillary Electrophoresis Coupled to Mass Spectrometry for Proteomic Profiling of Human Urine and Biomarker Discovery
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Chapter title
Capillary Electrophoresis Coupled to Mass Spectrometry for Proteomic Profiling of Human Urine and Biomarker Discovery
Chapter number 6
Book title
Proteomics
Published in
Methods in molecular biology, January 2009
DOI 10.1007/978-1-60761-157-8_6
Pubmed ID
19544019
Book ISBNs
978-1-60761-156-1, 978-1-60761-157-8
Authors

Petra Zürbig, Eric Schiffer, Harald Mischak, Zürbig, Petra, Schiffer, Eric, Mischak, Harald

Abstract

Currently, the main focus of clinical proteome analysis is on detection and identification of polypeptides that significantly change owing to pathological changes. Capillary electrophoresis coupled online to an electrospray ionization time of flight mass spectrometer (CE-MS) allows the differential display of a large number of polypeptides in a single, reproducible, and time-limited step and enables the comparison of different protein profiles for biomarker discovery. In addition to the reproducibility of the CE-MS setup, many further steps including data processing and mining, usage of biomarkers for diagnosis, and biomarker sequencing are necessary to answer the demands of biomarker discovery of clinical significance. In this chapter, we discuss materials and methods for CE-MS-based clinical proteomics allowing the reproducible profiling of urine.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
South Africa 1 5%
Unknown 21 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 27%
Researcher 4 18%
Professor 3 14%
Other 2 9%
Unspecified 2 9%
Other 3 14%
Unknown 2 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 36%
Biochemistry, Genetics and Molecular Biology 7 32%
Unspecified 2 9%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Computer Science 1 5%
Other 1 5%
Unknown 2 9%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 July 2014.
All research outputs
#18,374,472
of 22,758,248 outputs
Outputs from Methods in molecular biology
#7,869
of 13,089 outputs
Outputs of similar age
#157,560
of 169,015 outputs
Outputs of similar age from Methods in molecular biology
#139
of 159 outputs
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So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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