Basic Exercises in Immunochemistry

Table of Contents

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   Book Overview
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   Chapter 1 Fractionation of Serum Proteins by Ammonium Sulfate
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   Chapter 2 Isolation of Immunoglobulins by Gel Filtration on Sephadex G 200 Column
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   Chapter 3 Isolation of IgG by DEAE Cellulose
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   Chapter 4 Dissociation of Macroglobulins with 2-Mercaptoethanol
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   Chapter 5 Enzymatic Cleavage of IgG
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   Chapter 6 Chromatographic Separation of the IgG Fragments
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   Chapter 7 Separation and Isolation of H and L Chains of IgG
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   Chapter 8 Affinity Chromatography and Its Application to Antibody Isolation
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    Chapter 9 Cellulose Acetate Paper Electrophoresis of Human Serum
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    Chapter 10 Disc Electrophoresis in Polyacrylamide Gel
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    Chapter 11 Separation of Human Hemoglobins by Electrofocusing
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    Chapter 12 Conjugation of Antibodies with Fluorescein and Rhodamine
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    Chapter 13 Enzymatic Labeling of Proteins with 125 I
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    Chapter 14 Preparation of Peroxidase-Labeled Antibody
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    Chapter 15 Preparation of Soluble Dinitrophenyl Proteins
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    Chapter 16 Preparation of Dinitrophenyl- and Trinitrophenyl-Labeled Erythrocytes
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    Chapter 17 Isolation of Cell Membranes From Ascites Tumors
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    Chapter 18 Preparation and Use of Synthetic Liposomes
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    Chapter 19 Isolation of Murine Transplantation Antigen Preparation
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    Chapter 20 Isolation of Bacterial H-Antigens
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    Chapter 21 Extraction of Bacterial O Antigens (Endotoxins or lipopolysaccharides)
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    Chapter 22 Preparation of 14 C-Labeled Endotoxin and Endotoxic Glycolipid (EGL)
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    Chapter 23 Isolation of Bacterial Capsular Polysaccharides, K Antigens
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    Chapter 24 Extraction of Teichoic Acid
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    Chapter 25 Extraction of Pneumococcus Type XIV Polysaccharide
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    Chapter 26 Isolation of Phytohemagglutinin
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    Chapter 27 A and B Blood Group Antigen Preparations From Erythrocytes
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    Chapter 28 Isolation of NN Blood Group Antigen From Human Erythrocytes
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    Chapter 29 Isolation of Forssman Hapten From Erythrocytes
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    Chapter 30 Isolation of Ragweed Pollen Allergens
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    Chapter 31 Ultrasonic Disruption of Bacteria and Density-Gradient Centrifugation of the Products
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    Chapter 32 Vacuum Distillation and Freeze Drying
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    Chapter 33 Determination of Optimal Hydrolytic Conditions
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    Chapter 34 Hydrolysis of Lipopolysaccharide With Acid
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    Chapter 35 Partial Hydrolysis of Lipopolysaccharide With Ion Exchanger
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    Chapter 36 The Methods of Analytic and Preparative Paper Chromatography
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    Chapter 37 Separation and Isolation of Peptides or Oligosaccharides by High-Voltage Paper Electrophoresis
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    Chapter 38 Demonstration of Ion Exchange Column Chromatography of Amino Acids
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    Chapter 39 Gas-Liquid Chromatography for Fatty Acid Separation
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    Chapter 40 Carbohydrate Analysis by Gas-Liquid Chromatography
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    Chapter 41 Gas-Liquid Chromatographic Analysis of Amino Acids
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    Chapter 42 Demonstration of Thin-Layer Chromatography
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    Chapter 43 Qualitative Analysis of the Constituents of an Unknown Natural Product
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    Chapter 44 Identification of N-Terminal and C-Terminal Amino Acids
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    Chapter 45 Staining of Gels for Carbohydrates
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    Chapter 46 Methylation of Carbohydrates
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    Chapter 47 Dry Weight and Inorganic Ash Determinations
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    Chapter 48 Microdetermination of Nitrogen
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    Chapter 49 Microdetermination of Phosphorus
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    Chapter 50 Protein Determination by the Biuret Method
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    Chapter 51 Determination of Primary Amino Compounds with Ninhydrin
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    Chapter 52 Carbohydrate Determination by Phenol-Sulfuric Acid
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    Chapter 53 Ultramicrodetermination of Reducing Carbohydrates
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    Chapter 54 Enzymatic Determination of Carbohydrates
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    Chapter 55 Determination of Amino Sugars
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    Chapter 56 Determination of Heptoses and Pentoses
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    Chapter 57 Determination of Sialic Acid and 2-Keto-3-Deoxyoctonic Acid Derivatives
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    Chapter 58 Chemical and Biologic Analysis of Compounds Separated by Preparative Thin-Layer Chromatography
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    Chapter 59 Oxydation of Carbohydrates With Periodate. Quantitative Spectrophotometric Determination
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    Chapter 60 Measurements of the Products of Periodate Oxydation
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    Chapter 61 Reduction of Carbohydrates With NaBH 4
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    Chapter 62 Quantitative Determination of Free Hydroxyl Groups
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    Chapter 63 Lipid Determination With the Hydroxylamine Method
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    Chapter 64 Qualitative and Quantitative Analysis of Carboxylic Acids
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    Chapter 65 Immunization and Adjuvant Effect
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    Chapter 66 Demonstration of Antibody Production at Cellular Level (Immunoplaque Method)
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    Chapter 67 Bacterial Agglutination
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    Chapter 68 Hemagglutination and Its Inhibition
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    Chapter 69 Passive Hemagglutination and Its Inhibition
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    Chapter 70 Charcoal Agglutination
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    Chapter 71 Double Gel Diffusion/Ouchterlony Method
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    Chapter 72 Immunoelectrophoresis
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    Chapter 73 Quantitative Immunoelectrophoresis/Rocket Method
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    Chapter 74 Crossed Immunoelectrophoresis
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    Chapter 75 Counter Immunoelectrophoresis
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    Chapter 76 Semiquantitative Microprecipitin Assays
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    Chapter 77 Quantitative Precipitation
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    Chapter 78 Quantitative Radial Immunodiffusion
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    Chapter 79 Radioimmunoassays for Cyclic AMP and Cyclic GMP Determinations
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    Chapter 80 Solid-Phase Radioimmunoassay
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    Chapter 81 Complement Fixation
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    Chapter 82 Total and Viable Cell Counts
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    Chapter 83 Direct and Indirect Staining of Bacteria with Fluorescein-Labeled Antibodies
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    Chapter 84 Unlabeled Antibody-Enzyme Method to Localize Antigens
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    Chapter 85 Isolation of Human Lymphocytes by Sedimentation
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    Chapter 86 Isolation of Adhering Leukocytes
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    Chapter 87 Rosette-Forming Cell Assay
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    Chapter 88 Passive Cutaneous Anaphylaxis (PCA)
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    Chapter 89 Lymphoblast Assay
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    Chapter 90 T Cell-Mediated Cytotoxicity of Influenza Virus-Infected Cells
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    Chapter 91 The Local Shwartzman Phenomenon
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    Chapter 92 Measurement of the Activity of the Reticuloendothelial System
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    Chapter 93 Enhancement of Nonspecific Resistance to Infection
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    Chapter 94 Determination of Toxicity
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    Chapter 95 Measurement of Pyrogenicity